AMH ELISA kit (Mouse)

SKU: CEA228Mu

Size: 96Tests

# of Times Cited in literature: 25

Prepare Time: 1-3 days(please inquire for mutiple units)

Target Name: AMH

Target Full Name: Anti-Mullerian Hormone

Alternative Names: MIF; MIH; MIS; Müllerian Inhibiting Factor; Müllerian Inhibiting Hormone; Müllerian Inhibiting Substance

Target Species: Mouse

Uniprot: P27106

Gene ID: 11705

Featured Series: CE kit

Featured Series Function: Detects small molecule

Specificity: Reactive with Mouse AMH / Anti-Mullerian Hormone

Method: Colormetric

Detection principle: Competitive Inhibition

Detection range: 123.5-10,000pg/mL

Sensitivity: 50.2pg/mL

Assay Time: 2h

Sample Size: 50uL

Recommended/Predicted Sample Types: Serum, Plasma and other Biological Fluids

Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%

Reproducibility test menthod: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Anti-Mullerian Hormone (AMH) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Anti-Mullerian Hormone (AMH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100

Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)

Shelf-life: 12 months

Specificity: This assay has high sensitivity and excellent specificity for detection of Anti-Mullerian Hormone (AMH). No significant cross-reactivity or interference between Anti-Mullerian Hormone (AMH) and analogues was observed.

Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary: 1. Prepare all reagents, samples and standards; 2. Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C; 3. Aspirate and wash 3 times; 4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 5. Aspirate and wash 5 times; 6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 7. Add 50µL Stop Solution. Read at 450 nm immediately.

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Anti-Mullerian Hormone (AMH) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Anti-Mullerian Hormone (AMH) and unlabeled Anti-Mullerian Hormone (AMH) (Standards or samples) with the pre-coated antibody specific to Anti-Mullerian Hormone (AMH). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Anti-Mullerian Hormone (AMH) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Anti-Mullerian Hormone (AMH) in the sample.

Research Area: Endocrinology;Reproductive science;Hormone metabolism;

References Citing This Product: Long-term effects of methamphetamine exposure in adolescent mice on the future ovarian reserve in adulthood

Serum anti-mullerian hormone: Correlation with the ovarian follicular dynamics in healthy mice

Predictive accuracy of anti mullerian hormone as indicator of ovarian follicle loss in cyclophosphamide treated mice

Effect of controlled ovarian hyperstimulation on puberty and estrus in mice offspring

Rapamycin Prevents cyclophosphamide-induced Over-activation of Primordial Follicle pool through PI3K/Akt/mTOR Signaling Pathway in vivo.

Peroxiredoxin 2 deficiency accelerates age-related ovarian failure through the reactive oxygen species-mediated JNK pathway in mice

Prenatal exposure to polychlorinated biphenyl and umbilical cord hormones and birth outcomes in an island population

Dienogest suppresses the activation of primordial follicles and preserves the primordial follicle stockpile for fertility in mice

Involvement of obesity-associated upregulation of chemerin/chemokine-like receptor 1 in oxidative stress and apoptosis in ovaries and granulosa cells

Transplantation of umbilical cord–derived mesenchymal stem cells on a collagen scaffold improves ovarian function in a premature ovarian failure model of mice

Protective properties of glycogen synthase kinase-3 inhibition against doxorubicin-induced oxidative damage to mouse ovarian reserve

Menstrual blood derived mesenchymal stem cells combined with Bushen Tiaochong recipe improved chemotherapy-induced premature ovarian failure in …

Uterine Cells Improved Ovarian Function in a Murine Model of Ovarian Insufficiency

Basal characterization and in vitro differentiation of putative stem cells derived from the adult mouse ovary

After cyclophosphamide exposure, granulosa cells recover their anti©\M¨¹llerian hormone©\producing ability but not their numbers