AF/LE Purified Anti-Human/Mouse CD44 Antibody[IM7]

SKU: GBS-F11000

Size:1mg

Storage:Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

Shipping:Ice bag

Exp date:12 months

Category ID_II:Primary Antibodies

Category ID_III:Flow Cytometry Antibodies

Abbreviation:CD44

Target Synonym:MDU2; MDU3; MIC4;CD44;CD44 antigen;CDw44;Epican;LHR;PGP-1;PGP-I;Phagocytic glycoprotein 1;Phagocytic glycoprotein I

Research Areas:Cell Adhesion;Cell Biology;Immunology;Stem Cells

Conjugation:None (AF/LE)

Host:Rat

Species reactivity:Human;Mouse

Application:FCM

Usage:Each lot of this antibody is quality control tested by flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per 10⁶ cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Isotype:Rat IgG2b, κ

Clonality:Monoclonal

Clone NO.:IM7

UNIProt ID:P15379;P16070;

Background:CD44 is a 80-95 kD glycoprotein also known as Hermes, Pgp1, H-CAM, or HUTCH. It is expressed on all leukocytes, endothelial cells, hepatocytes, and mesenchymal cells. As B and T cells become activated or progress to the memory stage, CD44 expression increases from low or mid levels to high levels. Thus, CD44 has been reported to be a valuable marker for memory cell subsets. High CD44 expression on Treg cells has been associated with potent suppressive function via high production of IL-10. CD44 is an adhesion molecule involved in leukocyte attachment to and rolling on endothelial cells, homing to peripheral lymphoid organs and to the sites of inflammation, and leukocyte aggregation.

Concentration:≥ 1 mg/mL

Control catalog number:

Immunogen:Recombinant Human;Mouse CD44 protein

Buffer:Sterile PBS, pH 7.2. < 1.0 EU per mg of the antibody as determined by the LAL method.

Purification method:>98%, Protein A/G purified

Dilution:FCM 2 μg/mL(0.5×10⁶-1×10⁶ cells)

GeneID:12505;960