Ab1-42 ELISA kit (Rat)

SKU: CEA946Ra

Size: 96Tests

# of Times Cited in literature: 14

Prepare Time: 1-3 days(please inquire for mutiple units)

Target Name: Ab1-42

Target Full Name: Amyloid Beta Peptide 1-42

Alternative Names: -

Target Species: Rat

Uniprot: P08592

Gene ID: 54226

Featured Series: CE kit

Featured Series Function: Detects small molecule

Specificity: Reactive with Rat Ab1-42 / Amyloid Beta Peptide 1-42

Method: Colormetric

Detection principle: Competitive Inhibition

Detection range: 3.70-300pg/mL

Sensitivity: 1.61pg/mL

Assay Time: 2h

Sample Size: 50uL

Recommended/Predicted Sample Types: Serum, Plasma and other Biological Fluids

Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%

Reproducibility test menthod: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Amyloid Beta Peptide 1-42 (Ab1-42) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Amyloid Beta Peptide 1-42 (Ab1-42) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100

Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)

Shelf-life: 12 months

Specificity: This assay has high sensitivity and excellent specificity for detection of Amyloid Beta Peptide 1-42 (Ab1-42). No significant cross-reactivity or interference between Amyloid Beta Peptide 1-42 (Ab1-42) and analogues was observed.

Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary: 1. Prepare all reagents, samples and standards; 2. Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C; 3. Aspirate and wash 3 times; 4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 5. Aspirate and wash 5 times; 6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 7. Add 50µL Stop Solution. Read at 450 nm immediately.

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Amyloid Beta Peptide 1-42 (Ab1-42) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Amyloid Beta Peptide 1-42 (Ab1-42) and unlabeled Amyloid Beta Peptide 1-42 (Ab1-42) (Standards or samples) with the pre-coated antibody specific to Amyloid Beta Peptide 1-42 (Ab1-42). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Amyloid Beta Peptide 1-42 (Ab1-42) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Amyloid Beta Peptide 1-42 (Ab1-42) in the sample.

Research Area: Neuro science;

References Citing This Product: 慢病毒介导的ＣＢＳ基因过表达对原代皮质神经元

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Amyloid ? lowering and cognition enhancing effects of ghrelin receptor analog [D-Lys (3)] GHRP-6 in rat model of obesity

The Potential Effect of Epigallocatechin-3-Gallate alone or in Combination with Vitamin E and Selenium on Alzheimer's Disease Induced by Aluminum in Rats

芝麻素对高糖条件下PC12细胞Aβ和BACE-1表达的影响

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CART peptide activates the Nrf2/HO-1 antioxidant pathway and protects hippocampal neurons in a rat model of Alzheimer's disease

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Ex Vivo Investigation of Bexarotene and Nicotinamide Function as a Protect?ve Agent on Rat Synaptosomes Treated with A¦ (1-42)

The influence of vinpocetine alone or in combination with Epigallocatechin-3-gallate, Coenzyme COQ10, Vitamin E and Selenium as a potential neuroprotective …

Probiotics ameliorates LPS induced neuroinflammation injury on Aβ 1–42, APP, γ-β secretase and BDNF levels in maternal gut microbiota and fetal …