2-NBDG Glucose Uptake Cell-Based Kit

SKU: E-CK-A441

Size:200Assays

Storage:This product can be stored for 1 year at -20°C

Shipping:Gel Packs

Exp date:12 months

Category ID_I:Apoptosis and Cell Health Detection

Category ID_II:Cell Function Related Products

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Application:

Usage:Can be used for flow cytometry and fluorescence microscopy to detect the glucose uptake viability of cells; can also be used for the evaluation and screening of drugs for glucose metabolic diseases

Kit Type:Cell Function Related Products

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Detection Instrument:Fluorescence microscope;flow Cytometer

Tested Sample Types:Cell samples

Assay Time:2 hours

Detection Type:Fluorometric method; Flow cytometry

Background:

Test Principle:2-NBDG Reagent is a green fluorescently labeled deoxyglucose analog with the same transporters (GLUTs) and similar transport kinetics as D-glucose.It is allowed to be phosphorylated by hexokinase because of a modification at the C-2 position.When it enters the cell, it is phosphorylated by hexokinase at the C-6 position to produce 2-NBDG-6-phosphate (a fluorescent derivative). Thus the fluorescence intensity reflects the glucose uptake.

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