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In combination with an optimal buffer, the second generation of Topoisomerase, this product using a vector containing the suicide gene ccdB, when the insert is successfully ligated to the vector, the correct expression of ccdB is destroyed, and the host cell can grow normally, otherwise the host cell cannot grow normally, thereby achieving “zero” background. Containing a blunt end factor, 5min TA/Blunt-Zero Cloning Kit is compatible with both TA clones and blunt clones.
If you and your colleagues use both Taq, Pfu or another proof-reading polymerase in PCR, you do not need to worry about if you need to stock both the TA cloning kit and the blunt end cloning kit, or worry about using the wrong kit. Just keep this kit in the lab and have peace of mind in using this cloning kit for all your PCR cloning Needs.