VAHTS Total RNA-seq (H/M/R) Library Prep Kit for Illumina with rRNA depletion

The Vazyme VAHTS Total RNA-seq (H/M/R) Library Prep Kit for Illumina® is specially designed for the preparation of stranded transcriptome libraries for next generation sequencing (NGS) platforms of Illumina. 

Note: This kit, thus the price,  includes a module of rRNA depletion kit for human/mouse/rat DNA. 

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  • 24 reactions
  • 96 reactions

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The initial template is prepared with 0.1 µg-1 µg of total RNA of human, mouse, or rat. Compared with traditional transcriptome library preparation kits, this kit enables the depletion of ribosomal RNA (rRNA), including both cytoplasmic (28S, 18S, 5.8S, and 5S) and mitochondrial (16S and 12S) rRNA, leaving mRNA and other non-coding RNA behind. This kit is suitable for subsequent non-coding RNA analysis such as lncRNA. Degraded RNA (i.e. FFPE RNA) could also be used to prepare the library with this kit. In addition, this kit enables the insertion of dUTP during the 2nd strand synthesis of cDNA. The double strand cDNA are digested by uracil-DNA glycosylase (UDG) to remove the second strand before sequencing. As a result, only information from the 1st strand cDNA is preserved. In addition to standard transcriptome information, strand-specific (i.e. from sense or anti-sense DNA) information can also be obtained from NGS data analysis.

Kit Components:

ComponentsNR603-01 (24 rxn)NR603-02 (96 rxn)
NR 4rRNA Probe (H/M/R)24 μl96 μl
 Probe Buffer72 μl288 μl
 RNaseH Buffer24 μl96 μl
 RNaseH96 μl384 μl
 DNase I Buffer672 μl4 × 672 μl
 DNase I 48 μl192 μl
NR 5Frag/Prime Buffer444 μl2 × 888 μl
 Actinomycin D (5 mg/ml)24 μl96 μl
 1st Strand Buffer144 μl576 μl
 1st Strand Enzyme Mix48 μl192 μl
 2nd Strand Marking Buffer480 μl2 × 960 μl
 2nd Strand/End Repair Enzyme Mix120 μl480 μl
NR 6dA-Tailing Buffer Mix240 μl960 μl
 dA-Tailing Enzyme Mix60 μl240 μl
 Ligation Mix60 μl240 μl
 Stop Ligation Mix120 μl480 μl
 PCR Primer Mix120 μl480 μl
 Amplification Mix600 μl4 × 600 μl
 Heat-labile UDG24 μl96 μl

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Total RNA-seq lib Prep Kit

VAHTS Total RNA-seq (H/M/R) Library Prep Kit for Illumina with rRNA depletion

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