BL21 Chemically Competent Cell


BL21 Chemically Competent Cell is specifically designed for chemical transformation of DNA. It is resistant to TetR and permits a transformation efficiency of over 107 cfu/μg DNA (tested by pUC19 plasmid DNA).


E. coli B F- dcm omp T hsdS(rB-mB-) gal [malB+]K-12(λ S)

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  • 20 reactions
  • 40 reactions

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• Transformation efficiency: >107 cfu/μg (pUC19 DNA).

• TetR.

• Tight expression control ideal for toxic protein expression.

• Control plasmid II (Amp+) is used for detection of expression function of cell. The protein size is about 26 kDa.


at -70°C for six months. Do not store in liquid nitrogen

Papers citing the use of this product:

An autoinhibitory mechanism modulates MAVS activity in antiviral innate immune responseNATURE COMMUNICATIONS11.47Yuheng Shi, et al.2015 JulThe original link
A green strategy to immobilize silver nanoparticles onto reverse osmosis membrane for enhanced anti-biofouling propertydesalination5.5269Chenxi Dong,et al.2017JanThe original link
Substitutions in Spodoptera exigua topoisomerase I modulate its relaxation activity and camptothecin sensitivitypest management science3.25Pei Zhang et al State Key Laboratory for Biology o2017 JunThe original link
The essential roles of core binding factors CfRunt and CfCBF?? in hemocyte production of scallop Chlamys farreriDevelopmental & Comparative Immunology3.238Feng Yue, et al. Key Laboratory of Experimental Ma2014 JunThe original link
A teleost complement factor Ba possesses antimicrobial activity and inhibits bacterial infection in fishdevelopmental and comparative immunology3.2Li XP et al2017 JunThe original link

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BL21 Chemically Competent Cell

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