Size: 20 reactions (10x100ul; 50 ul per reaction)
BL21(DE3) Chemically Competent Cell is specifically designed for chemical transformation of DNA. It permits a transformation efficiency of over 107 cfu/μg DNA (tested by pUC19 plasmid DNA).
F- ompT hsdSB(rB- mB-) gal dcm lacY1 ahpC (DE3) gor522::Tn10 trxB (KanR,TetR)
This product is no longer in stock
• Transformation efficiency: >107 cfu/μg (pUC19 DNA).
• KanR and TetR.
• Thioredoxin reductase (trxB) and glutathione reductase (gor) mutation greatly facilitates cytoplasmic disulfide bond formation.
• Control plasmid I (Amp+) is used for detection of expression function of cell. The protein size is about 25 kDa.
at -70°C for six months. Do not store in liquid nitrogen
Papers citing the use of this product:
|Biochemical characteristics of New Delhi metallo-β-lactamase-1 show unexpected difference to other MBLs.||PLoS One||3.73||T Li, et al. State Key Laboratory of Pathogens and||2013 Apr||The original link|
|Expression and Characterization of a Recombinant Laccase with Alkalistable and Thermostable Properties from Streptomyces griseorubens JSD-1||Applied Biochemistry and Biotechnology||1.735||Haiwei Feng, et al.||2015 Mar||The original link|
|The lytic activity of recombinant phage lysin LysKΔamidase against staphylococcal strains associated with bovine and human infections in the Jiangsu province of China||research in veterinary science||1.2979||Yan Zhou,et al.||2017Apr||The original link|