TransB(DE3) Chemically Competent Cell


Size: 20 reactions (10x100ul; 50 ul per reaction)


BL21(DE3) Chemically Competent Cell is specifically designed for chemical transformation of DNA. It permits a transformation efficiency of over 107 cfu/μg DNA (tested by pUC19 plasmid DNA).


F- ompT hsdSB(rB- mB-) gal dcm lacY1 ahpC (DE3) gor522::Tn10 trxB (KanR,TetR

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• Transformation efficiency: >107 cfu/μg (pUC19 DNA).

• KanR and TetR.

• Thioredoxin reductase (trxB) and glutathione reductase (gor) mutation greatly facilitates cytoplasmic disulfide bond formation.

• Control plasmid I (Amp+) is used for detection of expression function of cell. The protein size is about 25 kDa.


at -70°C for six months. Do not store in liquid nitrogen

Papers citing the use of this product:

Biochemical characteristics of New Delhi metallo-β-lactamase-1 show unexpected difference to other MBLs.PLoS One3.73T Li, et al. State Key Laboratory of Pathogens and2013 AprThe original link
Expression and Characterization of a Recombinant Laccase with Alkalistable and Thermostable Properties from Streptomyces griseorubens JSD-1Applied Biochemistry and Biotechnology1.735Haiwei Feng, et al.2015 MarThe original link
The lytic activity of recombinant phage lysin LysKΔamidase against staphylococcal strains associated with bovine and human infections in the Jiangsu province of Chinaresearch in veterinary science1.2979Yan Zhou,et al.2017AprThe original link

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TransB(DE3) Chemically Competent Cell

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