BL21(DE3) pLysS Chemically Competent Cell is specifically designed for chemical transformation of DNA. It is resistant to CamR and permits a transformation efficiency of over 107 cfu/μg DNA (tested by pUC19 plasmid DNA).
F- ompT hsdS(rB-mB-)gal dcm(DE3)pLysS Camr
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• Transformation efficiency: >107 cfu/μg (pUC19 DNA).
• Contains pLysS plasmid that expresses the T7 lysozyme gene to reduce the background of the target protein expression without
disturbing IPTG functioning.
• Suitable for non-toxic and toxic protein’s expression.
• Control plasmid I (Amp+) is used for detection of expression function of cell. The protein size is about 25 kDa.
At -70°C for six months. Do not store in liquid nitrogen.
Papers cited the use of this product:
|A single fluorescent protein-based sensor for in vivo 2-oxogluatarate detection in cell||Biosensors and Bioelectronics||5.437||Chang Zhang, et al. Lab of Biosystems and Microana||2014 Apr||The original link|
|A Dimerization-Dependent Mechanism Drives the Endoribonuclease Function of PRRSV nsp11||J Virol.||4.439||Yuejun Shi, et al.||2016 Feb||The original link|
|Quantitative monitoring of 2-oxoglutarate in Escherichia coli cells by a fluorescence resonance energy transfer-based biosensor||Appl Microbiol Biotechnol||3.689||Chang Zhang, et al. Laboratory of Biosystems and M||2013 Jul||The original link|
|Amino acid residue Y196E substitution and C-terminal peptide synergistically alleviate the toxicity of Clostridium perfringens epsilon toxin||Toxicon||2.492||Wenwu Yao, et al.||2015 Apr||The original link|