BL21 (DE3) pLysS Chemically Competent Cell


BL21(DE3) pLysS Chemically Competent Cell is specifically designed for chemical transformation of DNA. It is resistant to CamR and permits a transformation efficiency of over 107 cfu/μg DNA (tested by pUC19 plasmid DNA).


F- ompT hsdS(rB-mB-)gal dcm(DE3)pLysS Camr

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  • 20 reactions
  • 40 reactions

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• Transformation efficiency: >107 cfu/μg (pUC19 DNA).

• CamR.

• Contains pLysS plasmid that expresses the T7 lysozyme gene to reduce the background of the target protein expression without

disturbing IPTG functioning.

• Suitable for non-toxic and toxic protein’s expression.

• Control plasmid I (Amp+) is used for detection of expression function of cell. The protein size is about 25 kDa.


At -70°C for six months. Do not store in liquid nitrogen.

Papers cited the use of this product:

A single fluorescent protein-based sensor for in vivo 2-oxogluatarate detection in cellBiosensors and Bioelectronics5.437Chang Zhang, et al. Lab of Biosystems and Microana2014 AprThe original link
A Dimerization-Dependent Mechanism Drives the Endoribonuclease Function of PRRSV nsp11J Virol.4.439Yuejun Shi, et al.2016 FebThe original link
Quantitative monitoring of 2-oxoglutarate in Escherichia coli cells by a fluorescence resonance energy transfer-based biosensorAppl Microbiol Biotechnol3.689Chang Zhang, et al. Laboratory of Biosystems and M2013 JulThe original link
Amino acid residue Y196E substitution and C-terminal peptide synergistically alleviate the toxicity of Clostridium perfringens epsilon toxinToxicon2.492Wenwu Yao, et al.2015 AprThe original link

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