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BL21 (DE3) pLysS Chemically Competent Cell
Description | BL21(DE3) pLysS Chemically Competent Cell is specifically designed for chemical transformation of DNA. It is resistant to CamR and permits a transformation efficiency of over 107 cfu/μg DNA (tested by pUC19 plasmid DNA). |
Genotype | F- ompT hsdS(rB-mB-)gal dcm(DE3)pLysS Camr |
More info
Features
• Transformation efficiency: >107 cfu/μg (pUC19 DNA).
• CamR.
• Contains pLysS plasmid that expresses the T7 lysozyme gene to reduce the background of the target protein expression without
disturbing IPTG functioning.
• Suitable for non-toxic and toxic protein’s expression.
• Control plasmid I (Amp+) is used for detection of expression function of cell. The protein size is about 25 kDa.
Storage
At -70°C for six months. Do not store in liquid nitrogen.
Papers cited the use of this product:
| Literature | Journal | IF | Author | Date | Link |
|---|---|---|---|---|---|
| A single fluorescent protein-based sensor for in vivo 2-oxogluatarate detection in cell | Biosensors and Bioelectronics | 5.437 | Chang Zhang, et al. Lab of Biosystems and Microana | 2014 Apr | The original link |
| A Dimerization-Dependent Mechanism Drives the Endoribonuclease Function of PRRSV nsp11 | J Virol. | 4.439 | Yuejun Shi, et al. | 2016 Feb | The original link |
| Quantitative monitoring of 2-oxoglutarate in Escherichia coli cells by a fluorescence resonance energy transfer-based biosensor | Appl Microbiol Biotechnol | 3.689 | Chang Zhang, et al. Laboratory of Biosystems and M | 2013 Jul | The original link |
| Amino acid residue Y196E substitution and C-terminal peptide synergistically alleviate the toxicity of Clostridium perfringens epsilon toxin | Toxicon | 2.492 | Wenwu Yao, et al. | 2015 Apr | The original link |
