pEASY®-Blunt E1 Expression Kit


Size: 20 reactions (10 x 100 ul)

pEASY®-Blunt E1 Expression Vector is constructed from pET vector, it utilizes a highly efficient, five-minute blunt cloning strategy to clone PCR product into high-efficient expression vector. The size of control insert is 750 bp, and expressed target protein is about 27 kDa.

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• 5 minutes fast ligation of Pfu-amplified PCR products.

• Ampicillin resistance.

• T7 promoter primer and T7 terminator primer for sequencing.

• Bacteriophage T7lac promoter for high level expression.

• N-terminal 6×His tag for easy purification.

• Trans1-T1 phage resistant chemically competent cell, high transformation efficiency (>109 cfu/μg pUC19 DNA) and fast growing.

• E1 Expression Plasmid included as negative control.


Competent Cell at -70°C for six months; others at -20°C for nine months

Kit Content


CE111-01 (10 rxns)

pEASY®-Blunt E1 Expression Vector (15 ng/μl)

10 µl

E1 Expression Plasmid (Negative Control) (15 ng/μl)

10 µl

EControl Template (5 ng/μl)

10 µl

EControl Forward Primer (10 μM)

10 µl

EControl Reverse Primer (10 μM)

10 µl

T7 Promoter Primer (10 μM)

50 µl

T7 Terminator Primer (10 μM)

50 µl

Trans1-T1 Phage Resistant Chemically Competent Cell

5×100 μl

Papers citing this product:

A Viral Satellite DNA Vector (TYLCCNV) for Functional Analysis of miRNAs and siRNAs in PlantsPlant Physiology7.367Dongyan Cao, et al. College of Food Science and Nu2017 AprThe original link
Identification of a novel class B scavenger receptor homologue in Portunus trituberculatus: Molecular cloning and microbial ligand bindingfish & shellfish immunology3.025Yang N, et al. Key Laboratory of the Ministry of E2016 NovThe original link
Genome-wide identification and functional analysis of S-RNase involved in the self-incompatibility of citrusmolecular genetics and genomics2.622Liang M, et al. Key Laboratory of Horticultural Pl2016 DecThe original link
Functional characterization of aphid ORinsect science2.026Rui-Bin Zhang,et al.2017JulThe original link
Characterization of a neutral recombinant xylanase from Thermoactinospora rubra YIM 77501Tantonie van leeuwenhoek international journal of g1.944Yin YR, et al. Yunnan Institute of Microbiology, Y2017 MarThe original link

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