See Below for Detailed Description
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Updated Date: Stock Protein updated on 20171228
Research areas: Others
Target / Protein: vvhA
Biologically active: Not Tested
Expression system: E.coli
Species of origin: Vibrio vulnificus (strain CMCP6)
Delivery time: 3-7 business days
Uniprot ID: P19247
AA Sequence: QEYVPIVEKPIYITSSKIKCVLHTSGDFNATRDWCNAGASIDVRVNVAQMRSVQSATSDGFTPDAKIVRFTVDADKPGTGIHLVNELQQDHSWFQSWANRRTYIGPFASSYDLWVKPVSGYTPKKARDLPQNENKNYQHRDTYGYSIGINGKVGAEVNKDGPKVGGEVSGSFTYNYSKTLVFDTKDYRINNRSSLSDFDISFEREFGECDELRRQELGCYFTAAHWGSGWVFDKTKFNPISYSNFKPNYDVLYEAPVSETGVTDFEMGVKLNYRARFGTVLPSALFSVYGSAGSSTNSSTVKQRIRIDWNHPLFEAEAHVTLQSLSNNDLCLDVYGENGDKTVAGGSVNGWSCHGSWNQVWGLDKEERYRSRVASDRCLTVNADKTLTVEQCGANLAQKWYWEGDKLISRYVDGNNTRYLLNIVGGRNVQVTPENEANQARWKPTLQQVKL
Tag info: N-terminal 10xHis-tagged and C-terminal Myc-tagged
Expression Region: 21-471aa
Protein length: Full Length of Mature Protein
MW: 55.8 kDa
Relevance: Bacterial hemolysins are exotoxins that attack blood cell membranes and cause cell rupture by mechanisms not clearly defined.
Reference: "The cytolysin gene of Vibrio vulnificus: sequence and relationship to the Vibrio cholerae E1 Tor hemolysin gene." Yamamoto K., Wright A.C., Kaper J.B., Morris J.G. Jr. Infect. Immun. 58:2706-2709(1990)
Purity: Greater than 85% as determined by SDS-PAGE.
Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.