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Updated Date: Stock Protein updated on 20171018
Research areas: Others
Target / Protein: VP30
Biologically active: Not Tested
Expression system: E.coli
Species of origin: Zaire ebolavirus (strain Kikwit-95) (ZEBOV) (Zaire Ebola virus)
Delivery time: 3-7 business days
Uniprot ID: Q77DJ5
AA Sequence: MEASYERGRPRAARQHSRDGHDHHVRARSSSRENYRGEYRQSRSASQVRVPTVFHKKRVEPLTVPPAPKDICPTLKKGFLCDSSFCKKDHQLESLTDRELLLLIARKTCGSVEQQLNITAPKDSRLANPTADDFQQEEGPKITLLTLIKTAEHWARQDIRTIEDSKLRALLTLCAVMTRKFSKSQLSLLCETHLRREGLGQDQAEPVLEVYQRLHSDKGGSFEAALWQQWDRQSLIMFITAFLNIALQLPCESSAVVVSGLRTLVPQSDNEEASTNPGTCSWSDEGTP
Tag info: N-terminal 6xHis-tagged
Expression Region: 1-288aa
Protein length: Full Length
MW: 38.0 kDa
Alternative Name(s): Transcription activator VP30
Relevance: Acts as a transcription anti-termination factor immediately after transcription initiation, but does not affect transcription elongation. This function has been found to be dependent on the formation of an RNA stem-loop at the transcription start site of the first gene. Binds to RNA
Reference: "Crystal structure of the C-terminal domain of Ebola virus VP30 reveals a role in transcription and nucleocapsid association." Hartlieb B., Muziol T., Weissenhorn W., Becker S. Proc. Natl. Acad. Sci. U.S.A. 104:624-629(2007)
Purity: Greater than 90% as determined by SDS-PAGE.
Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.