See Below for Detailed Description
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Updated Date: Stock Protein updated on 20170725
Research areas: Neuroscience
Target / Protein: Blm
Biologically active: Not Tested
Expression system: E.coli
Species of origin: Mus musculus (Mouse)
Delivery time: 3-7 business days
Uniprot ID: O88700
AA Sequence: INAALLGEDCFILMPTGGGKSLCYQLPACVSPGVTIVISPLRSLIVDQVQKLTSFDIPATYLTGDKTDSEAANIYLQLSKKDPIIKLLYVTPEKVCASNRLISTLENLYERKLLARFVIDEAHCVSQWGHDFRQDYKRMNMLRQKFPSVPVMALTATANPRVQKDILTQLKILRPQ
Tag info: N-terminal 10xHis-tagged and C-terminal MYC-tagged
Expression Region: 684-859aa
Protein length: Partial
MW: 24.7 kDa
Alternative Name(s): RecQ helicase homolog
Relevance: Participates in DNA replication and repair. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction. Involved in 5'-end resection of DNA during double-strand break (DSB) repair: unwinds DNA and recruits DNA2 which mediates the cleavage of 5'-ssDNA. Negatively regulates sister chromatid exchange (SCE)
Reference: "Point mutations causing Bloom's syndrome abolish ATPase and DNA helicase activities of the BLM protein." Bahr A., de Graeve F., Kedinger C., Chatton B. Oncogene 17:2565-2571(1998)
Purity: Greater than 90% as determined by SDS-PAGE.
Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.