See Below for Detailed Description
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Updated Date: Stock Protein updated on 20170405
Research areas: Microbiology
Target / Protein: nlpD
Biologically active: Not Tested
Expression system: E.coli
Species of origin: Escherichia coli (strain K12)
Delivery time: 3-7 business days
Uniprot ID: P0ADA3
AA Sequence: CSDTSNPPAPVSSVNGNAPANTNSGMLITPPPKMGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR
Tag info: N-terminal 6xHis-SUMO-tagged
Expression Region: 26-379aa
Protein length: Full Length
MW: 53.5 kDa
Relevance: Activator of the cell wall hydrolase AmiC. Required for septal murein cleavage and daughter cell separation during cell division.
Reference: "LytM-domain factors are required for daughter cell separation and rapid ampicillin-induced lysis in Escherichia coli."Uehara T., Dinh T., Bernhardt T.G.J. Bacteriol. 191:5094-5107(2009).
Purity: Greater than 90% as determined by SDS-PAGE.
Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.