{"product_id":"transscript-r-ii-multiplex-probe-one-step-qrt-pcr-supermix-udg","title":"TransScript® II Multiplex Probe One-Step qRT-PCR SuperMix UDG","description":"\u003cdiv class=\"page-title anchor\" data-anchor=\"introduce\"\u003e\n\u003ch3\u003eProduct Details\u003c\/h3\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"product-brief\"\u003e\n\u003cp\u003e\u003cspan\u003e\u003cem\u003eTransScript\u003c\/em\u003e\u003csup\u003e®\u003c\/sup\u003e II Multiplex Probe One-Step qRT-PCR SuperMix UDG is designed for one-step qRT-PCR with high sensitivity, high synthesis efficiency and high amplification efficiency. This kit firstly synthesizes first-strand cDNA with RNA as templates using reverse gene-specific primers, and then performs qPCR with the synthesized cDNA as templates using both forward and reverse gene-specific primers and fluorescent probes to achieve one step from reverse transcription to qPCR in a single tube. After special optimization, this kit enables consistently amplifying multiple targets in a single reaction ensuring multiplex one-step RT-qPCR. dUTP\/ UDG is included in the kit to degrade dU-containing ssDNA and dsDNA, which can prevent cross contamination.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eHighlights\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• Use \u003cem\u003eT\u003c\/em\u003e\u003cem\u003eransScript\u003c\/em\u003e\u003csup\u003e®\u003c\/sup\u003e II Multiplex Probe One-Step Enzyme Mix UDG, 2×\u003cem\u003ePerfectStart\u003c\/em\u003e\u003csup\u003e®\u003c\/sup\u003e Multiplex Probe One-Step Reaction Mix to efficiently synthesize RNA into first-strand cDNA for qPCR. It is easy to operate and reduces the chance of contamination during operation. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• Using UDG enzyme and dUTP to effectively prevent cross-contamination of PCR products with accurate data. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• High sensitivity, high specificity and the data are accurate.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• Singleplex to 4-plex RT-qPCR detection.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• High-copy and low-copy gene detection.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• RNA templates with high GC content or complex secondary structure.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• Detection of RNA virus or trace amounts of RNA.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eStore at -20°C for two years\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eShipping\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eDry ice (-70 ℃)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"region-title\"\u003eProduct Composition\u003c\/div\u003e\n\u003cdiv class=\"text\"\u003e\n\u003ctable border=\"1\" width=\"-103\"\u003e\n\u003ctbody\u003e\n\u003ctr class=\"firstRow\"\u003e\n\u003ctd\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003eAQ322-01\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003eAQ322-02\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cem\u003eTransScript\u003c\/em\u003e\u003csup\u003e®\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003eII Multiplex Probe One-Step Enzyme Mix UDG\u003csup\u003e\u003cbr\u003e\u003c\/sup\u003e\n\u003c\/td\u003e\n\u003ctd\u003e80 μl\u003c\/td\u003e\n\u003ctd\u003e320 μl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e2×\u003cem\u003ePerfectStart\u003c\/em\u003e\u003csup\u003e®\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003eMultiplex Probe One-Step Reaction Mix\u003c\/td\u003e\n\u003ctd\u003e1 ml\u003c\/td\u003e\n\u003ctd\u003e4×1 ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePassive Reference Dye (50×)\u003c\/td\u003e\n\u003ctd\u003e40 μl\u003c\/td\u003e\n\u003ctd\u003e160 μl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eNuclease-free Water\u003c\/td\u003e\n\u003ctd\u003e1 ml\u003c\/td\u003e\n\u003ctd\u003e4×1 ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e","brand":"Gene Bio Systems","offers":[{"title":"100 rxns×20 μl system","offer_id":46787575185508,"sku":"AQ322-01","price":334.88,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/1_6681fc76-aaa2-4a4e-9cf9-cdaf4fdfac5a.jpg?v=1756484266","url":"https:\/\/www.genebiosystems.com\/products\/transscript-r-ii-multiplex-probe-one-step-qrt-pcr-supermix-udg","provider":"GeneBio ","version":"1.0","type":"link"}