{"product_id":"transngs-r-single-cell-full-length-cdna-synthesis-amplification-kit","title":"TransNGS® Single Cell Full Length cDNA Synthesis \u0026 Amplification Kit","description":"\u003cdiv class=\"product-brief\"\u003e\n\u003cp\u003e\u003cspan\u003eTransNGS\u003csup\u003e®\u003c\/sup\u003e Single Cell Full Length cDNA Synthesis \u0026amp; Amplification Kit is a reagent kit capable of obtaining  amplified full-length cDNA from single cell. It is suitable for amplifying and constructing cDNA libraries from  1-500 cells or 10 pg-10 ng total RNA. The kit uses Single Cell Oligo (dT) as the reverse transcription primer and  employs a reverse transcriptase with high synthesis efficiency and template-switching activity to add a special  sequence to the 3' end of the cDNA, thereby obtaining full-length cDNA products. The kit is compatible with  various cell types and tissue types, suitable for cell materials with varying RNA content. Typically, a single-cell  library can yield 10-60 ng of amplified cDNA products.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch1 class=\"product-content-sub-title\"\u003eFeatures\u003c\/h1\u003e\n\u003cp\u003e• Strong compatibility with different cell types, suitable for cells with low RNA content (such as immune cells). \u003c\/p\u003e\n\u003cp\u003e• Strong compatibility with different tissue types, suitable for tissues that are difficult to dissociate (such as brain tissue). \u003c\/p\u003e\n\u003cp\u003e• High yield in constructing single-cell libraries, excellent peak shapes, high efficiency in gene detection (FPKM \u0026gt; 1)\u003c\/p\u003e\n\u003ch1 class=\"product-content-sub-title\"\u003eStorage\u003c\/h1\u003e\n\u003cp\u003e\u003cspan\u003eSingle cell TSO should be stored at -70℃ for one year. The other components should be stored at -20℃  for one year\u003c\/span\u003e\u003c\/p\u003e\n\u003ch1 class=\"product-content-sub-title\"\u003eShipping\u003c\/h1\u003e\n\u003cp\u003e\u003cspan\u003eDry ice (-70℃)\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca rel=\"noopener\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/KC901-Single_Cell_Full_Length_cDNA_syn_and_amp_kit-GBS.pdf?v=1763472815\" target=\"_blank\"\u003e\u003cspan\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\" alt=\"\" width=\"188\" height=\"53\"\u003e\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ch1 class=\"region-title\"\u003eProduct Composition\u003c\/h1\u003e\n\u003cdiv class=\"text\"\u003e\n\u003ctable border=\"1\" width=\"670\"\u003e\n\u003ctbody\u003e\n\u003ctr class=\"firstRow\"\u003e\n\u003ctd\u003e\n\u003cp\u003eComponent\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" valign=\"null\" width=\"null\"\u003eKC901-01 (12 rxns) \u003c\/td\u003e\n\u003ctd\u003eKC901-02 (24 rxns)\u003c\/td\u003e\n\u003ctd\u003eKC901-03 (96 rxns)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSingle Cell Lysis Buffer \u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" valign=\"null\" width=\"null\"\u003e12 μl \u003c\/td\u003e\n\u003ctd\u003e24 μl \u003c\/td\u003e\n\u003ctd\u003e96 μl \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\"\u003eSingle Cell Oligo(dT) \u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\"\u003e12 μl \u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\"\u003e24 μl \u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\"\u003e96 μl \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\"\u003e10 mM dNTPs \u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\"\u003e12 μl \u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\"\u003e24 μl \u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\"\u003e96 μl \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\"\u003eRibonuclease Inhibitors \u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\"\u003e5 μl \u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\"\u003e10 μl \u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\"\u003e40 μl \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\"\u003eSingle Cell RT Buffer \u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\"\u003e24 μl \u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\"\u003e48 μl \u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\"\u003e192 μl \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDTT \u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" valign=\"null\" width=\"null\"\u003e12 μl \u003c\/td\u003e\n\u003ctd\u003e24 μl \u003c\/td\u003e\n\u003ctd\u003e96 μl \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSingle Cell TSO \u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" valign=\"null\" width=\"null\"\u003e6 μl \u003c\/td\u003e\n\u003ctd\u003e12 μl \u003c\/td\u003e\n\u003ctd\u003e48 μl \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" valign=\"null\"\u003eSingle Cell RT Enhancer \u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" valign=\"null\"\u003e6 μl \u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" valign=\"null\"\u003e12 μl \u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" valign=\"null\"\u003e48 μl \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" valign=\"null\"\u003eSingle Cell Reverse Transcriptase \u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" valign=\"null\"\u003e12 μl \u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" valign=\"null\"\u003e24 μl \u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" valign=\"null\"\u003e96 μl \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" valign=\"null\"\u003e2×Single Cell Amplification SuperMix \u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" valign=\"null\"\u003e300 μl \u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" valign=\"null\"\u003e600 μl \u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" valign=\"null\"\u003e2.4 ml \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" valign=\"null\"\u003eSingle Cell PCR Primer \u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" valign=\"null\"\u003e12 μl \u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" valign=\"null\"\u003e24 μl \u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" valign=\"null\"\u003e96 μl \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" valign=\"null\"\u003eRNase-free Water \u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" valign=\"null\"\u003e1 ml \u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" valign=\"null\"\u003e2 ml \u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" valign=\"null\"\u003e5 ml \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eLibrary Elution Buffer\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" valign=\"null\" width=\"null\"\u003e300 μl \u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd\u003e600 μl \u003c\/td\u003e\n\u003ctd\u003e2.4 ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cp\u003e\u003cbr\u003e\u003cbr\u003e\u003cbr\u003e\u003cbr\u003e\u003cbr\u003e\u003cbr\u003e\u003cbr\u003e\u003cbr\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"12 rxns","offer_id":46084325113956,"sku":"KC901-01","price":1186.08,"currency_code":"CAD","in_stock":true},{"title":"24 rxns","offer_id":46084325146724,"sku":"KC901-02","price":2238.88,"currency_code":"CAD","in_stock":true},{"title":"96 rxns","offer_id":46084325179492,"sku":"KC901-03","price":5218.08,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/1_ec51cb5a-c23f-44fa-aec0-b975aa783b88.jpg?v=1750702741","url":"https:\/\/www.genebiosystems.com\/products\/transngs-r-single-cell-full-length-cdna-synthesis-amplification-kit","provider":"GeneBio ","version":"1.0","type":"link"}