{"product_id":"rfwd3-polyclonal-antibody-ab","title":"RFWD3 Polyclonal Antibody","description":"\u003cp\u003e\u003cb\u003eSize\u003c\/b\u003e:100μL\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBackground\u003c\/b\u003e:RFWD3, also named as RNF201, is a E3 ubiquitin-protein ligase that mediates the ubiquitination of p53\/TP53 in the late response to DNA damage, and acts as a positive regulator of p53\/TP53 stability, thereby regulating the G1\/S DNA damage checkpoint. RFWD3 may act by catalyzing the formation of short polyubiquitin chains on p53\/TP53 that are not targeted to the proteasome. In response to ionizing radiation, It interacts with MDM2 and enhances p53\/TP53 ubiquitination, possibly by restricting MDM2 from extending polyubiquitin chains on ubiquitinated p53\/TP53. (PMID: 20173098 ) From BLAST result, this antibody is specific to RFWD3. 45kd and 55kd maybe splicesome of RFWD3.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eResearch_area\u003c\/b\u003e:Others\u003c\/p\u003e\u003cp\u003e\u003cb\u003eTarget_protein\u003c\/b\u003e:RFWD3\u003c\/p\u003e\u003cp\u003e\u003cb\u003eapplications\u003c\/b\u003e:WB, IHC-p, ELISA\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReactivity\u003c\/b\u003e:Human\u003c\/p\u003e\u003cp\u003e\u003cb\u003eClonality\u003c\/b\u003e:Polyclonal\u003c\/p\u003e\u003cp\u003e\u003cb\u003eClone ID\u003c\/b\u003e:\u003c\/p\u003e\u003cp\u003e\u003cb\u003eHost\u003c\/b\u003e:Rabbit\u003c\/p\u003e\u003cp\u003e\u003cb\u003eIsotype\u003c\/b\u003e:IgG\u003c\/p\u003e\u003cp\u003e\u003cb\u003eGene Symbol\/ Name\u003c\/b\u003e:RFWD3 Antibody\u003c\/p\u003e\u003cp\u003e\u003cb\u003eImmunogen\u003c\/b\u003e:The antiserum was produced against synthesized peptide derived from human AMPK alpha around the phosphorylation site of Thr172. AA range:140-189\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage\u003c\/b\u003e:-20°C for 1 year\u003c\/p\u003e\u003cp\u003e\u003cb\u003ePurification\u003c\/b\u003e:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormulation\u003c\/b\u003e:Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eConcentration\u003c\/b\u003e:1 mg\/ml\u003c\/p\u003e\u003cp\u003e\u003cb\u003eMolecular weight(Da)\u003c\/b\u003e:85094\u003c\/p\u003e\u003cp\u003e\u003cb\u003eUniProt accession\u003c\/b\u003e:Human: Q6PCD5; Mouse: Q8CIK8\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSynonyms\u003c\/b\u003e:RFWD3; RNF201; E3 ubiquitin-protein ligase RFWD3; RING finger and WD repeat domain-containing protein 3; RING finger protein 201\u003c\/p\u003e\u003cp\u003e\u003cb\u003eGeneID\u003c\/b\u003e:Human: 55159\u003c\/p\u003e","brand":"GeneBio Systems","offers":[{"title":"Default Title","offer_id":48649694281828,"sku":"BT-AP07780","price":693.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/no_image_default_image-jpeg_735ebede-c8dc-4d4c-bd38-ccb992e1e19e.jpg?v=1782724164","url":"https:\/\/www.genebiosystems.com\/products\/rfwd3-polyclonal-antibody-ab","provider":"GeneBio ","version":"1.0","type":"link"}