{"product_id":"recombinant-mouse-tnf-alpha-protein","title":"Recombinant Mouse TNF- Alpha protein","description":"\u003cp\u003e\u003cb\u003eSize\u003c\/b\u003e:5 ug\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBackground\u003c\/b\u003e:Tumor necrosis factor alpha (TNF-α), also called cachectin, is the best-know member of the TNF-family, which can cause cell death. This protein is produced by neutrophils, activated lymphocytes, macrophages, NK cells, LAK cells, astrocytes endothelial cells, smooth muscle cells and some transformed cells. TNF-α occurs as a secreted, soluble form and as a membrane-anchored form, both of which are biologically active. The naturally-occurring form of TNF-α is glycosylated, but non-glycosylated recombinant TNF-α has comparable biological activity. The biologically active native form of TNF-α is reportedly a trimer. Human and Mouse TNF-α show approximately 79 % homology at the amino acid level and cross-reactivity between the two species. Two types of receptors for TNF-α have been described and virtually all cell types studied show the presence of one or both of these receptor types.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eResearch_area\u003c\/b\u003e:Immunology\u003c\/p\u003e\u003cp\u003e\u003cb\u003ePurity\u003c\/b\u003e:\u0026gt; 98 % by SDS-PAGE and HPLC analyses.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSource\u003c\/b\u003e:Escherichia coli.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFull product name\u003c\/b\u003e:Recombinant Mouse Tumor Necrosis Factor-alpha\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBiological activity\u003c\/b\u003e:Fully biologically active when compared to standard. The ED5 as determined by a cytotoxicity assay using Mouse L929 cells is less than .1 ng\/ml, corresponding to a specific activity of \u0026gt; 1. × 17 IU\/mg in the presence of actinomycin D.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSynonyms\u003c\/b\u003e:TNF-α,DI, DIF, TNF-, TNF-a, TNF-alpha, TNFSF2, TNFalpha, Tna, Tnfs, Tnfsf1a, Tnlg1f, Tnf, Mouse\u003c\/p\u003e\u003cp\u003e\u003cb\u003eEndotoxin\u003c\/b\u003e:Less than 1 EU\/μg of rMuTNF-α as determined by LAL method.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage\u003c\/b\u003e:-20ºC\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSequence\u003c\/b\u003e:MLRSSSQNSS DKPVAHVVAN HQVEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLVY SQVLFKGQGC PDYVLLTHTV SRFAISYQEK VNLLSAVKSP CPKDTPEGAE LKPWYEPIYL GGVFQLEKGD QLSAEVNLPK YLDFAESGQV YFGVIAL\u003c\/p\u003e\u003cp\u003e\u003cb\u003euniprot accession\u003c\/b\u003e:P684\u003c\/p\u003e\u003cp\u003e\u003cb\u003eMolecular weight\u003c\/b\u003e:Approximately 17.4 kDa. The recombinant Mouse TNF-α is a soluble 157 amino acid protein which corresponds to C-terminal extracellular domain of the full length transmembrane protein.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormulation\u003c\/b\u003e:\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReconstitution\u003c\/b\u003e:Lyophilized from a .2 μm filtered solution in PBS, pH 7.2.\u003c\/p\u003e","brand":"GeneBio Systems","offers":[{"title":"Default Title","offer_id":48653943832676,"sku":"CD00195","price":473.09,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/no_image_default_image-jpeg_e1e7a0a7-0461-448e-af51-f038c67008b9.jpg?v=1782765625","url":"https:\/\/www.genebiosystems.com\/products\/recombinant-mouse-tnf-alpha-protein","provider":"GeneBio ","version":"1.0","type":"link"}