{"product_id":"recombinant-mouse-fgf-8-protein","title":"Recombinant Mouse FGF-8 protein","description":"\u003cp\u003e\u003cb\u003eSize\u003c\/b\u003e:5 ug\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBackground\u003c\/b\u003e:Mouse FGF-8 is a heparin binding growth factor belonging to the FGF family, which plays a central role during prenatal development, postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. Mouse FGF-8 is first purified from an androgen-dependent mouse mammary carcinoma cell line as an androgen induces secretion. Cloning and analysis of the Mouse FGF8 gene revealed at least eight potential protein isoforms (FGF-8 a-h). Mouse FGF-8a and b share 100 % amino acid identity with that in humans, and Mouse FGF-8e and f share 98 % amino acid identity with humans. None of the FGF-8 isoforms exhibited activity to FGFR1b, 2b, 3b, but FGFR2c, 3c and FGFR4 can be activated by several FGF-8 isoforms. FGF-8 plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration, and it is required for normal brain, eye, ear and limb development during embryogenesis.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eResearch_area\u003c\/b\u003e:Immunology\u003c\/p\u003e\u003cp\u003e\u003cb\u003ePurity\u003c\/b\u003e:\u0026gt; 97 % by SDS-PAGE and HPLC analyses.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSource\u003c\/b\u003e:Escherichia coli.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFull product name\u003c\/b\u003e:Recombinant Mouse Fibroblast Growth Factor-8\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBiological activity\u003c\/b\u003e:Fully biologically active when compared to standard. The ED5 as determined by a cell proliferation assay using Mouse balb\/c 3T3 cells is less than 5. ng\/ml, corresponding to a specific activity of \u0026gt; 2. × 15 IU\/mg in the presence of 1 μg\/ml of heparin.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSynonyms\u003c\/b\u003e:FGF-8,Ai, Aigf, Fgf8, Fgf6c, Mouse\u003c\/p\u003e\u003cp\u003e\u003cb\u003eEndotoxin\u003c\/b\u003e:Less than 1EU\/μg of rMuFGF-8 as determined by LAL method.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage\u003c\/b\u003e:-20ºC\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSequence\u003c\/b\u003e:QVRSAAQKRG PGAGNPADTL GQGHEDRPFG QRSRAGKNFT NPAPNYPEEG SKEQRDSVLP KVTQRHVREQ SLVTDQLSRR LIRTYQLYSR TSGKHVQVLA NKRINAMAED GDPFAKLIVE TDTFGSRVRV RGAETGLYIC MNKKGKLIAK SNGKGKDCVF TEIVLENNYT ALQNAKYEGW YMAFTRKGRP RKGSKTRQHQ REVHFMKRLP RGHHTTEQSL RFEFLNYPPF TR\u003c\/p\u003e\u003cp\u003e\u003cb\u003euniprot accession\u003c\/b\u003e: P37237\u003c\/p\u003e\u003cp\u003e\u003cb\u003eMolecular weight\u003c\/b\u003e:Approximately 28.1 kDa, a single non-glycosylated polypeptide chain containing 246 amino acids.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormulation\u003c\/b\u003e:\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReconstitution\u003c\/b\u003e:Lyophilized from a .2 μm filtered concentrated solution in PBS, pH 7.4, 5 mM NaCl.\u003c\/p\u003e","brand":"GeneBio Systems","offers":[{"title":"Default Title","offer_id":48653956120676,"sku":"CD00453","price":473.09,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/no_image_default_image-jpeg_05d1bc41-61c0-4db1-b2d7-8c298498b992.jpg?v=1782765988","url":"https:\/\/www.genebiosystems.com\/products\/recombinant-mouse-fgf-8-protein","provider":"GeneBio ","version":"1.0","type":"link"}