{"product_id":"recombinant-mouse-14-3-3-sigma-ywhas-protein","title":"Recombinant Mouse 14-3-3 sigma\/YWHAS Protein","description":"\u003cp\u003e\u003cb\u003eSize\u003c\/b\u003e: 100μg\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage\u003c\/b\u003e: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eShipping\u003c\/b\u003e: This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eExp date\u003c\/b\u003e: 12 months\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCategory ID_II\u003c\/b\u003e: Recombinant Proteins\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCategory ID_III\u003c\/b\u003e: Others\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAbbreviation\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eTarget Synonym\u003c\/b\u003e: Er;Mme1;Ywhas\u003c\/p\u003e\u003cp\u003e\u003cb\u003eResearch Areas\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eConjugation\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eTarget Species\u003c\/b\u003e: Mouse\u003c\/p\u003e\u003cp\u003e\u003cb\u003eExpression Host\u003c\/b\u003e: E.coli\u003c\/p\u003e\u003cp\u003e\u003cb\u003eApplication\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eFusion tag\u003c\/b\u003e: None\u003c\/p\u003e\u003cp\u003e\u003cb\u003eUNIProt ID\u003c\/b\u003e: O70456\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAccession\u003c\/b\u003e: NP_061224.2\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBackground\u003c\/b\u003e: 14-3-3 protein sigma (YWHAS), also known as stratifin  (SFN) and epithelial cell marker protein 1, is a member of the14-3-3 proteins  which are a family of  conserved regulatory molecules expressed in all eukaryotic cells. The name 14-3-3 refers to the  particular elution and migration pattern of these proteins on DEAE-cellulose  chromatography and starch-gel electrophoresis. The 14-3-3 proteins eluted in  the 14th fraction of bovine brain homogenate and were found on positions 3.3 of  subsequent electrophoresis. There are seven genes that encode 14-3-3s in most  mammals. 14-3-3 proteins have been identified as adapter proteins implicated in the regulation of a large  spectrum of both general and specialized signaling pathway. More than 100 signaling proteins have  been reported as 14-3-3 ligands including kinases, phosphatases, and  transmembrane receptors, and the binding generally results in the modulation of the  activity of the binding partner. YWHAE exists as a homodimer and present mainly in tissues enriched in  stratified squamous keratinising epithelium. YWHAS  has been repoted to interact with KRT17 and GAB2, and may regulate protein  synthesis and epithelial cell growth by stimulating Akt\/mTOR pathway upon  binding to KRT17. Additionally, YWHAS (SFN) may also act as a p53-regulated  inhibitor of G2\/M progression.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eConcentration\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eActivity\u003c\/b\u003e: Not validated for activity\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSequence\u003c\/b\u003e: Met 1-Ser 248\u003c\/p\u003e\u003cp\u003e\u003cb\u003ePurity\u003c\/b\u003e:  \u0026gt; 95 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormulation\u003c\/b\u003e: Lyophilized from sterile 50mM Tris, 150mM NaCl, pH 8.0\u003cbr\u003eNormally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.\u003cbr\u003ePlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReconstitution\u003c\/b\u003e: Please refer to the printed manual for detailed information.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eEndotoxin\u003c\/b\u003e: Please contact us for more information.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCalculated MW\u003c\/b\u003e: 27.7 kDa\u003c\/p\u003e\u003cp\u003e\u003cb\u003eObservedMW\u003c\/b\u003e: 32 kDa\u003c\/p\u003e","brand":"GeneBio Systems","offers":[{"title":"Default Title","offer_id":47986037194852,"sku":"PKSM040602","price":1224.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/no_image_default_image-jpeg_862b5a49-acee-439a-a4ca-d1e414961130.jpg?v=1772480581","url":"https:\/\/www.genebiosystems.com\/products\/recombinant-mouse-14-3-3-sigma-ywhas-protein","provider":"GeneBio ","version":"1.0","type":"link"}