{"product_id":"recombinant-human-pcsk9-protein-d374y-his-tag","title":"Recombinant Human PCSK9 Protein (D374Y, His Tag)","description":"\u003cp\u003e\u003cb\u003eSize\u003c\/b\u003e: 50μg\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage\u003c\/b\u003e: Store at \u0026lt; -20°C, stable for 6 months. Please minimize freeze-thaw cycles.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eShipping\u003c\/b\u003e: This product is provided as liquid. It is shipped at frozen temperature with blue ice\/gel packs. Upon receipt, store it immediately at \u0026lt; - 20°C.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eExp date\u003c\/b\u003e: 12 months\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCategory ID_II\u003c\/b\u003e: Recombinant Proteins\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCategory ID_III\u003c\/b\u003e: Others\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAbbreviation\u003c\/b\u003e: PCSK9\u003c\/p\u003e\u003cp\u003e\u003cb\u003eTarget Synonym\u003c\/b\u003e: NARC-1;NARC1;Neural Apoptosis-Regulated Convertase 1;PC9;PCSK9;Proprotein Convertase 9;Proprotein Convertase Subtilisin\/Kexin Type 9;Subtilisin\/Kexin-Like Protease PC9\u003c\/p\u003e\u003cp\u003e\u003cb\u003eResearch Areas\u003c\/b\u003e: Signal Transduction;Cell biology;Cardiovascular;Cancer;metabolism;Stem cells\u003c\/p\u003e\u003cp\u003e\u003cb\u003eConjugation\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eTarget Species\u003c\/b\u003e: Human\u003c\/p\u003e\u003cp\u003e\u003cb\u003eExpression Host\u003c\/b\u003e: HEK293 Cells\u003c\/p\u003e\u003cp\u003e\u003cb\u003eApplication\u003c\/b\u003e: ELISA\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFusion tag\u003c\/b\u003e: C-His\u003c\/p\u003e\u003cp\u003e\u003cb\u003eUNIProt ID\u003c\/b\u003e: Q8NBP7\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAccession\u003c\/b\u003e: Q8NBP7\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBackground\u003c\/b\u003e: Recombinant Human Proprotein Convertase Subtilisin\/Kexin Type 9\/PCSK9 (D374Y) is a gain of function mutant of human PCSK9 protein. Human PCSK9 is a secretory subtilase belonging to the proteinase K subfamily. PCSK9 is synthesized as a soluble zymogen that undergoes autocatalytic intramolecular processing in the ER, the pro domain and mature chain are secreted together through noncovalent interactions. PCSK9 binds with low-density lipoprotein receptor (LDLR) and it plays a major regulatory role in cholesterol homeostasis. Inhibition of PCSK9 function by preventing PCSK9\/LDLR interaction is currently being explored as a means of lowering cholesterol levels. PCSK9 also binds to apolipoprotein receptor 2 (ApoER2), and play a role in the neural development.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eConcentration\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eActivity\u003c\/b\u003e: Immobilized Recombinant Human LDL R (C-Fc) at 5μg\/ml (100 μl\/well) can bind Recombinant Human PCSK9 . The ED\u003csub\u003e50\u003c\/sub\u003e of Recombinant Human PCSK9 is 39.17 ng\/ml.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSequence\u003c\/b\u003e: Gln31-Gln692(Asp374Tyr,Val474Ile,Gly504Arg,Gly670Glu)\u003c\/p\u003e\u003cp\u003e\u003cb\u003ePurity\u003c\/b\u003e:  \u0026gt; 90 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormulation\u003c\/b\u003e: Supplied as a 0.2 μm filtered solution of 50mM HEPES, 150mM NaCl, 20% Glycerol, pH 7.4.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReconstitution\u003c\/b\u003e: Not Applicable\u003c\/p\u003e\u003cp\u003e\u003cb\u003eEndotoxin\u003c\/b\u003e:  \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCalculated MW\u003c\/b\u003e: 15-18\u0026amp;60-80 kDa\u003c\/p\u003e\u003cp\u003e\u003cb\u003eObservedMW\u003c\/b\u003e: 13.8\u0026amp;58.2 kDa\u003c\/p\u003e","brand":"GeneBio Systems","offers":[{"title":"Default Title","offer_id":47982472527972,"sku":"PKSH032947","price":356.4,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/no_image_default_image-jpeg_76a88065-9257-41b8-89ba-42f3530210f1.jpg?v=1772385837","url":"https:\/\/www.genebiosystems.com\/products\/recombinant-human-pcsk9-protein-d374y-his-tag","provider":"GeneBio ","version":"1.0","type":"link"}