{"product_id":"recombinant-human-pcsk1-nec1-protein-his-tag","title":"Recombinant Human PCSK1\/NEC1 Protein (His Tag)","description":"\u003cp\u003e\u003cb\u003eSize\u003c\/b\u003e: 500μg\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage\u003c\/b\u003e: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eShipping\u003c\/b\u003e: This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eExp date\u003c\/b\u003e: 12 months\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCategory ID_II\u003c\/b\u003e: Recombinant Proteins\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCategory ID_III\u003c\/b\u003e: Others\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAbbreviation\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eTarget Synonym\u003c\/b\u003e: BMIQ12;NEC1;PC1;PC3;SPC3\u003c\/p\u003e\u003cp\u003e\u003cb\u003eResearch Areas\u003c\/b\u003e: Signal Transduction;Cell biology;Neuroscience;Cancer;metabolism;\u003c\/p\u003e\u003cp\u003e\u003cb\u003eConjugation\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eTarget Species\u003c\/b\u003e: Human\u003c\/p\u003e\u003cp\u003e\u003cb\u003eExpression Host\u003c\/b\u003e: HEK293 Cells\u003c\/p\u003e\u003cp\u003e\u003cb\u003eApplication\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eFusion tag\u003c\/b\u003e: C-His\u003c\/p\u003e\u003cp\u003e\u003cb\u003eUNIProt ID\u003c\/b\u003e: P29120\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAccession\u003c\/b\u003e: NP_000430.3\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBackground\u003c\/b\u003e: Neuroendocrine convertase 1, also  known as Prohormone convertase 1, Proprotein convertase 1, PCSK1 and NEC1, is  anenzyme which belongs to thepeptidase S8 family and Furin  subfamily. PCSK1 is an enzyme that performs the proteolytic cleavage of  prohormones to their intermediate (or sometimes completely cleaved) forms. It  is present only in neuroendocrine cells such as brain, pituitary and adrenal,  and most often cleaves after a pair of basic residues within prohormones but  can occasionally cleave after a single arginine. It binds to a protein known as  proSAAS, which also represents its endogenous inhibitor. PCSK1 is involved in  the processing of hormone and other protein precursors at sites comprised of  pairs of basic amino acid residues. PCSK1 substrates include POMC, renin,  enkephalin, dynorphin, somatostatin and insulin. Defects in PCSK1 are the cause  of proprotein convertase 1 deficiency (PC1 deficiency). PC1 deficiency is  characterized by obesity, hypogonadism, hypoadrenalism, reactive hypoglycemia  as well as marked small-intestinal absorptive dysfunction. It is due to  impaired processing of prohormones.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eConcentration\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eActivity\u003c\/b\u003e: Not validated for activity\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSequence\u003c\/b\u003e: Met 1-Arg 617\u003c\/p\u003e\u003cp\u003e\u003cb\u003ePurity\u003c\/b\u003e:  \u0026gt; 95 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormulation\u003c\/b\u003e: Lyophilized from sterile 25mM Tris, 150mM NaCl, pH 7.5\u003cbr\u003eNormally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.\u003cbr\u003ePlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReconstitution\u003c\/b\u003e: Please refer to the printed manual for detailed information.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eEndotoxin\u003c\/b\u003e:  \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCalculated MW\u003c\/b\u003e: 57.4 kDa\u003c\/p\u003e\u003cp\u003e\u003cb\u003eObservedMW\u003c\/b\u003e: 66 kDa\u003c\/p\u003e","brand":"GeneBio Systems","offers":[{"title":"Default Title","offer_id":47978845339748,"sku":"PKSH031846","price":6408.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/no_image_default_image-jpeg_d6181694-b9e2-4095-93f3-74ff0a9ad3c2.jpg?v=1772293375","url":"https:\/\/www.genebiosystems.com\/products\/recombinant-human-pcsk1-nec1-protein-his-tag","provider":"GeneBio ","version":"1.0","type":"link"}