{"product_id":"recombinant-human-otub1-otb1-protein-his-tag","title":"Recombinant Human OTUB1\/OTB1 Protein (His Tag)","description":"\u003cp\u003e\u003cb\u003eSize\u003c\/b\u003e: 100μg\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage\u003c\/b\u003e: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eShipping\u003c\/b\u003e: This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eExp date\u003c\/b\u003e: 12 months\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCategory ID_II\u003c\/b\u003e: Recombinant Proteins\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCategory ID_III\u003c\/b\u003e: Others\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAbbreviation\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eTarget Synonym\u003c\/b\u003e: HSPC263;OTB1;OTU1\u003c\/p\u003e\u003cp\u003e\u003cb\u003eResearch Areas\u003c\/b\u003e: Epigenetics and Nuclear Signaling,Cell Biology\u003c\/p\u003e\u003cp\u003e\u003cb\u003eConjugation\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eTarget Species\u003c\/b\u003e: Human\u003c\/p\u003e\u003cp\u003e\u003cb\u003eExpression Host\u003c\/b\u003e: E.coli\u003c\/p\u003e\u003cp\u003e\u003cb\u003eApplication\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eFusion tag\u003c\/b\u003e: N-His\u003c\/p\u003e\u003cp\u003e\u003cb\u003eUNIProt ID\u003c\/b\u003e: Q96FW1\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAccession\u003c\/b\u003e: Q96FW1-1\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBackground\u003c\/b\u003e: Ubiquitin  thioesterase OTUB1, also known as Deubiquitinating enzyme OTUB1, OTU  domain-containing ubiquitin aldehyde-binding protein 1, Otubain-1,  Ubiquitin-specific-processing protease OTUB1, OTUB1 and OTB1, is a cytoplasm  protein which belongs to thepeptidase C65 family. OTUB1 is a hydrolase  that can remove conjugated ubiquitin from proteins and plays an important  regulatory role at the level of protein turnover by preventing degradation.  OTUB1 is a regulator of T-cell anergy, a phenomenon that occurs when T-cells  are rendered unresponsive to antigen rechallenge and no longer respond to their  cognate antigen. OTUB1 acts via its interaction with RNF128 \/ GRAIL, a crucial  inductor of CD4 T-cell anergy. Isoform1 of OTUB1 destabilizes RNF128,  leading to prevent anergy. In contrast, isoform2of OTUB1 stabilizes  RNF128 and promotes anergy. OTUB1 regulates RNF128-mediated ubiquitination, but  does not deubiquitinate polyubiquitinated RNF128. Deubiquitinates estrogen  receptor alpha (ESR1). OTUB1 mediates deubiquitination of 'Lys-48'-linked  polyubiquitin chains, but not 'Lys-63'-linked polyubiquitin chains. OTUB1 is  also capable of removing NEDD8 from NEDD8 conjugates, but with a much lower  preference compared to 'Lys-48'-linked ubiquitin.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eConcentration\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eActivity\u003c\/b\u003e: Not validated for activity\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSequence\u003c\/b\u003e: Met 1-Lys 271\u003c\/p\u003e\u003cp\u003e\u003cb\u003ePurity\u003c\/b\u003e:  \u0026gt; 97 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormulation\u003c\/b\u003e: Lyophilized from sterile PBS, 20% glycerol, pH 7.4\u003cbr\u003eNormally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.\u003cbr\u003ePlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReconstitution\u003c\/b\u003e: Please refer to the printed manual for detailed information.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eEndotoxin\u003c\/b\u003e: Please contact us for more information.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCalculated MW\u003c\/b\u003e: 32.8 kDa\u003c\/p\u003e\u003cp\u003e\u003cb\u003eObservedMW\u003c\/b\u003e: 37 kDa\u003c\/p\u003e","brand":"GeneBio Systems","offers":[{"title":"Default Title","offer_id":47972846305380,"sku":"PKSH030767","price":590.4,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/no_image_default_image-jpeg_3411589c-b3ba-4083-b7a6-f43f0e73e34e.jpg?v=1772200075","url":"https:\/\/www.genebiosystems.com\/products\/recombinant-human-otub1-otb1-protein-his-tag","provider":"GeneBio ","version":"1.0","type":"link"}