{"product_id":"recombinant-human-ltc4s-ltc4-synthase-protein-his-tag","title":"Recombinant Human LTC4S\/LTC4 synthase Protein (His Tag)","description":"\u003cp\u003e\u003cb\u003eSize\u003c\/b\u003e: 50μg\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage\u003c\/b\u003e: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eShipping\u003c\/b\u003e: This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eExp date\u003c\/b\u003e: 12 months\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCategory ID_II\u003c\/b\u003e: Recombinant Proteins\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCategory ID_III\u003c\/b\u003e: Others\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAbbreviation\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eTarget Synonym\u003c\/b\u003e: LTC4S;MGC33147\u003c\/p\u003e\u003cp\u003e\u003cb\u003eResearch Areas\u003c\/b\u003e: Signal Transduction;Cancer;immunology;metabolism;\u003c\/p\u003e\u003cp\u003e\u003cb\u003eConjugation\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eTarget Species\u003c\/b\u003e: Human\u003c\/p\u003e\u003cp\u003e\u003cb\u003eExpression Host\u003c\/b\u003e: Baculovirus-Insect Cells\u003c\/p\u003e\u003cp\u003e\u003cb\u003eApplication\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eFusion tag\u003c\/b\u003e: C-His\u003c\/p\u003e\u003cp\u003e\u003cb\u003eUNIProt ID\u003c\/b\u003e: Q16873\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAccession\u003c\/b\u003e: NP_665874.1\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBackground\u003c\/b\u003e: Leukotriene  C4 synthase, also known as LTC4 synthase, Leukotriene-C(4) synthase, and LTC4S,  is a multi-pass membrane protein which belongs to theMAPEG family. LTC4S  is detected in lung, platelets and the myelogenous leukemia cell line KG-1 (at  protein level). LTC4S activity is present in eosinophils, basophils, mast  cells, certain phagocytic mononuclear cells, endothelial cells, vascular smooth  muscle cells and platelets. LTC4S is essential for the production of cysteinyl  leukotrienes (Cys-LT), critical mediators in asthma. Mutagenic analysis of the  conjugation function of human LTC4S has identified R51 and Y93 as critical for  acid and base catalysis of LTA4 and reduced glutathione, respectively. A  comparison across species for proteins that possess LTC4S activity reveals  conservation of both of these residues, whereas R51 is absent in the FLAP  molecules. Thus, within the glutathione S-transferase superfamily of genes,  alignment of specific residues allows the separation of LTC4S family members from  their most structurally similar counterparts, the FLAP molecules. Defects in  LTC4S are the cause of leukotriene C4 synthase deficiency (LTC4 synthase  deficiency). LTC4 synthase deficiency is a fatal neurometabolic developmental  disorder. It is associated with muscular hypotonia, psychomotor retardation,  failure to thrive, and microcephaly.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eConcentration\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eActivity\u003c\/b\u003e: Not validated for activity\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSequence\u003c\/b\u003e: Met 1-Ala 150\u003c\/p\u003e\u003cp\u003e\u003cb\u003ePurity\u003c\/b\u003e:  \u0026gt; 90 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormulation\u003c\/b\u003e: Lyophilized from sterile 50mM Hepes, 0.1% Triton 0.5% DOC, 10% glycerol, pH 8.0\u003cbr\u003eNormally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.\u003cbr\u003ePlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReconstitution\u003c\/b\u003e: Please refer to the printed manual for detailed information.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eEndotoxin\u003c\/b\u003e:  \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCalculated MW\u003c\/b\u003e: 17 kDa\u003c\/p\u003e\u003cp\u003e\u003cb\u003eObservedMW\u003c\/b\u003e: 17 kDa\u003c\/p\u003e","brand":"GeneBio Systems","offers":[{"title":"Default Title","offer_id":47972974526564,"sku":"PKSH031060","price":1224.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/no_image_default_image-jpeg_7e234854-ce6f-4422-948e-c2c00d020b0c.jpg?v=1772201619","url":"https:\/\/www.genebiosystems.com\/products\/recombinant-human-ltc4s-ltc4-synthase-protein-his-tag","provider":"GeneBio ","version":"1.0","type":"link"}