{"product_id":"recombinant-human-lr3-igf-1-protein","title":"Recombinant Human LR3 IGF-1 protein","description":"\u003cp\u003e\u003cb\u003eSize\u003c\/b\u003e:1 mg\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBackground\u003c\/b\u003e:IGF-1 belonged to the insulin gene family, is a mitogenic polypeptide growth factor that stimulate the proliferation and survival of various cell types including muscle, bone, and cartilage tissue in vitro. It is produced primarily by the liver as an endocrine hormone as well as in target tissues in a paracrine\/autocrine fashion. The production of IGF-1 is stimulated by growth hormone (GH) and can be retarded by undernutrition, growth hormone insensitivity, lack of growth hormone receptors, or failures of the downstream signaling pathway post GH receptor including SHP2 and STAT5B. The LR3 IGF-1 is a long-term analog of human IGF-1, specifically designed and manufactured for mammalian cell culture to support large-scale manufacturing of recombinant biopharmaceuticals.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eResearch_area\u003c\/b\u003e:Immunology\u003c\/p\u003e\u003cp\u003e\u003cb\u003ePurity\u003c\/b\u003e:\u0026gt;98 % by SDS-PAGE analyses.\u0026gt;90 % by RP-HPLC analyses.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSource\u003c\/b\u003e:Escherichia coli.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFull product name\u003c\/b\u003e:Recombinant Human LR3 Insulin-like Growth Factor-1\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBiological activity\u003c\/b\u003e:Assay 1: Fully biologically active when compared to standard. Measured in a serum-free cell proliferation assay using human MCF-7 cells. The ED5 for this effect is .3-1.5 ng\/ml, corresponding to a specific activity of \u0026gt; 6.7 × 15 IU\/mg.Assay 2: Fully biologically active when compared to standard. The ED5 as determined by the stimulation of protein synthesis using rat L6 myoblasts is less than 1 ng\/ml, corresponding to a specific activity of \u0026gt; 1. × 15 IU\/mg.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSynonyms\u003c\/b\u003e:LR3 IGF-1, IGF, IGF-I, IGFI, MGF,Human\u003c\/p\u003e\u003cp\u003e\u003cb\u003eEndotoxin\u003c\/b\u003e:Less than 0.01 EU\/μg of rHuLR3 IGF-1 as determined by LAL method.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage\u003c\/b\u003e:-20ºC\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSequence\u003c\/b\u003e:MFPAMPLSSL FVNGPRTLCG AELVDALQFV CGDRGFYFNK PTGYGSSSRR APQTGIVDEC CFRSCDLRRL EMYCAPLKPA KSA\u003c\/p\u003e\u003cp\u003e\u003cb\u003euniprot accession\u003c\/b\u003e:P519\u003c\/p\u003e\u003cp\u003e\u003cb\u003eMolecular weight\u003c\/b\u003e:Approximately 9.1 kDa, a single non-glycosylated polypeptide chain containing 83 amino acids.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormulation\u003c\/b\u003e:\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReconstitution\u003c\/b\u003e:Lyophilized from a .2 μm filtered concentrated solution in 2 mM PB, pH 7.2.\u003c\/p\u003e","brand":"GeneBio Systems","offers":[{"title":"Default Title","offer_id":48653999472740,"sku":"CD01368","price":553.73,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/no_image_default_image-jpeg_977c3b7e-babb-4712-ae47-0a2e80ad4856.jpg?v=1782767329","url":"https:\/\/www.genebiosystems.com\/products\/recombinant-human-lr3-igf-1-protein","provider":"GeneBio ","version":"1.0","type":"link"}