{"product_id":"recombinant-human-lap-tgf-beta-1","title":"Recombinant Human LAP (TGF-beta 1)","description":"\u003cp\u003e\u003cb\u003eSize\u003c\/b\u003e:10ug\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDescription\u003c\/b\u003e:Recombinant Human Transforming Growth Factor beta 1 is produced by our Mammalian expression system and the target gene encoding Leu30-Arg278(Cys33Ser) is expressed.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAccession\u003c\/b\u003e:P01137\u003c\/p\u003e\u003cp\u003e\u003cb\u003eMolecular weight\u003c\/b\u003e:28.5 KDa\u003c\/p\u003e\u003cp\u003e\u003cb\u003eApparent molecular weight\u003c\/b\u003e:20-30 KDa, reducing conditions\u003c\/p\u003e\u003cp\u003e\u003cb\u003eOther names\u003c\/b\u003e:Transforming Growth Factor Beta-1; TGF-Beta-1; Latency-Associated Peptide; LAP; TGFB1; TGFB\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage condition\u003c\/b\u003e:Lyophilized protein should be stored at \u0026lt; -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20°C for 3 months.\u003c\/p\u003e\u003cp\u003e\u003cb\u003ePurity\u003c\/b\u003e:Greater than 95% as determined by reducing SDS-PAGE.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eEndotoxin\u003c\/b\u003e:Less than 0.1 ng\/µg (1 EU\/µg) as determined by LAL test.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBiological activity\u003c\/b\u003e:\u003c\/p\u003e\u003cp\u003e\u003cb\u003eRedissolve\u003c\/b\u003e:Always centrifuge tubes before opening.Do not mix by vortex or pipetting.\nIt is not recommended to reconstitute to a concentration less than 100μg\/ml.\nDissolve the lyophilized protein in distilled water.\nPlease aliquot the reconstituted solution to minimize freeze-thaw cycles. \u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage\u003c\/b\u003e:Lyophilized protein should be stored at \u0026lt; -20°C, though stable at room temperature for 3 weeks.\nReconstituted protein solution can be stored at 4-7°C for 2-7 days.\nAliquots of reconstituted samples are stable at \u0026lt; -20°C for 3 months.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDelivery condition\u003c\/b\u003e:The product is shipped at ambient temperature.\nUpon receipt, store it immediately at the temperature listed below.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBackground\u003c\/b\u003e:Transforming Growth Factor β-1 (TGFβ-1) is a secreted protein which belongs to the TGF-β family. TGFβ-1 is abundantly expressed in bone, articular cartilage and chondrocytes and is increased in osteoarthritis (OA). TGFβ-1 performs many cellular functions, including the control of cell growth, cell proliferation, cell differentiation and apoptosis. The precursor is cleaved into a latency-associated peptide (LAP) and a mature TGFβ-1 peptide.Disulfide-linked homodimers of LAP and TGF-beta 1 remain non-covalently associated after secretion, forming the small latent TGF-beta 1 complex. Purified LAP is also capable of associating with active TGF-beta with high affinity, and can neutralize TGF-beta activity. Covalent linkage of LAP to one of three latent TGF-beta binding proteins (LTBPs) creates a large latent complex that may interact with the extracellular matrix. TGF-beta activation from latency is controlled both spatially and temporally, by multiple pathways that include actions of proteases such as plasmin and MMP9, and\/or by thrombospondin 1 or selected integrins. Although different isoforms of TGF-beta are naturally associated with their own distinct LAPs, the TGF-beta 1 LAP is capable of complexing with, and inactivating, all other human TGF-beta isoforms and those of most other species. Mutations within the LAP are associated with Camurati-Engelmann disease, a rare sclerosing bone dysplasia characterized by inappropriate presence of active TGF-beta 1.\u003c\/p\u003e","brand":"GeneBio Systems","offers":[{"title":"Default Title","offer_id":48647903215716,"sku":"EPT048","price":356.16,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/no_image_default_image-jpeg_f2d225e1-b059-4df9-bc09-1e89a9b73d8c.jpg?v=1782704407","url":"https:\/\/www.genebiosystems.com\/products\/recombinant-human-lap-tgf-beta-1","provider":"GeneBio ","version":"1.0","type":"link"}