{"product_id":"recombinant-human-itch-aip4-protein-aa-526-903","title":"Recombinant Human ITCH\/AIP4 Protein (aa 526-903)","description":"\u003cp\u003e\u003cb\u003eSize\u003c\/b\u003e: 100μg\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage\u003c\/b\u003e: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eShipping\u003c\/b\u003e: This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eExp date\u003c\/b\u003e: 12 months\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCategory ID_II\u003c\/b\u003e: Recombinant Proteins\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCategory ID_III\u003c\/b\u003e: Others\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAbbreviation\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eTarget Synonym\u003c\/b\u003e: ADMFD;AIF4;AIP4;NAPP1;dJ468O1.1\u003c\/p\u003e\u003cp\u003e\u003cb\u003eResearch Areas\u003c\/b\u003e: Cell biology\u003c\/p\u003e\u003cp\u003e\u003cb\u003eConjugation\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eTarget Species\u003c\/b\u003e: Human\u003c\/p\u003e\u003cp\u003e\u003cb\u003eExpression Host\u003c\/b\u003e: E.coli\u003c\/p\u003e\u003cp\u003e\u003cb\u003eApplication\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eFusion tag\u003c\/b\u003e: None\u003c\/p\u003e\u003cp\u003e\u003cb\u003eUNIProt ID\u003c\/b\u003e: Q96J02\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAccession\u003c\/b\u003e: NP_113671.3\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBackground\u003c\/b\u003e: E3 ubiquitin-protein ligase Itchy  homolog, also known as Atrophin-1-interacting protein 4, NFE2-associated  polypeptide 1, NAPP1 and ITCH, is a cell membrane protein which contains  oneC2 domain, oneHECT (E6AP-type E3 ubiquitin-protein ligase) domain  and contains fourWW domains. ITCH acts as an E3 ubiquitin-protein ligase  which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of  a thioester and then directly transfers the ubiquitin to targeted substrates.  It catalyzes 'Lys-29'-, 'Lys-48'- and 'Lys-63'-linked ubiquitin conjugation.  ITCH is involved in the control of inflammatory signaling pathways. It is an  essential component of a ubiquitin-editing protein complex, comprising also  TNFAIP3, TAX1BP1 and RNF11, that ensures the transient nature of inflammatory  signaling pathways. ITCH promotes the association of the complex after TNF  stimulation. Once the complex is formed, TNFAIP3 deubiquitinates 'Lys-63'  polyubiquitin chains on RIPK1 and catalyzes the formation of 'Lys-48'-polyubiquitin  chains. This leads to RIPK1 proteosomal degradation and consequently  termination of the TNF- or LPS-mediated activation of NFKB1. Defects in ITCH  are the cause of syndromic multisystem autoimmune disease (SMAD) which is  characterized by organomegaly, failure to thrive, developmental delay,  dysmorphic features and autoimmune inflammatory cell infiltration of the lungs,  liver and gut.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eConcentration\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eActivity\u003c\/b\u003e: Not validated for activity\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSequence\u003c\/b\u003e: Arg 526-Glu 903\u003c\/p\u003e\u003cp\u003e\u003cb\u003ePurity\u003c\/b\u003e:  \u0026gt; 98 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormulation\u003c\/b\u003e: Lyophilized from sterile 20mM Tris, 200mM NaCl, 10% glycerol, pH 8.0\u003cbr\u003eNormally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.\u003cbr\u003ePlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReconstitution\u003c\/b\u003e: Please refer to the printed manual for detailed information.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eEndotoxin\u003c\/b\u003e: Please contact us for more information.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCalculated MW\u003c\/b\u003e: 40 kDa\u003c\/p\u003e\u003cp\u003e\u003cb\u003eObservedMW\u003c\/b\u003e: 40 kDa\u003c\/p\u003e","brand":"GeneBio Systems","offers":[{"title":"Default Title","offer_id":47973013717092,"sku":"PKSH031249","price":1368.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/no_image_default_image-jpeg_fb2c8e3b-e549-4b79-be9b-be1bf2e5983f.jpg?v=1772202585","url":"https:\/\/www.genebiosystems.com\/products\/recombinant-human-itch-aip4-protein-aa-526-903","provider":"GeneBio ","version":"1.0","type":"link"}