{"product_id":"recombinant-human-ilkap-protein-his-tag","title":"Recombinant Human ILKAP Protein (His Tag)","description":"\u003cp\u003e\u003cb\u003eSize\u003c\/b\u003e: 100μg\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage\u003c\/b\u003e: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eShipping\u003c\/b\u003e: This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eExp date\u003c\/b\u003e: 12 months\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCategory ID_II\u003c\/b\u003e: Recombinant Proteins\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCategory ID_III\u003c\/b\u003e: Others\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAbbreviation\u003c\/b\u003e: ILKAP\u003c\/p\u003e\u003cp\u003e\u003cb\u003eTarget Synonym\u003c\/b\u003e: ILKAP;ILKAP2;ILKAP3;PP2C-DELTA;PPM1O;DKFZp434J2031;FLJ10181;Integrin linked kinase associated phosphatase;Integrin linked kinase associated serine\/threonine phosphatase;Integrin linked kinase associated serine\/threonine phosphatase 2C;Integrin-linked kinase-associated serine\/threonine phosphatase 2C;MGC4846;PP2C DELTA;Protein phosphatase 2c delta isozyme\u003c\/p\u003e\u003cp\u003e\u003cb\u003eResearch Areas\u003c\/b\u003e: Signal Transduction;\u003c\/p\u003e\u003cp\u003e\u003cb\u003eConjugation\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eTarget Species\u003c\/b\u003e: Human\u003c\/p\u003e\u003cp\u003e\u003cb\u003eExpression Host\u003c\/b\u003e: HEK293 Cells\u003c\/p\u003e\u003cp\u003e\u003cb\u003eApplication\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eFusion tag\u003c\/b\u003e: C-His\u003c\/p\u003e\u003cp\u003e\u003cb\u003eUNIProt ID\u003c\/b\u003e: Q9H0C8\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAccession\u003c\/b\u003e: Q9H0C8\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBackground\u003c\/b\u003e: Integrin-linked  kinase-associated serine\/threonine phosphatase 2C, also known as ILKAP, is  cytoplasm protein which belongs to thePP2C family. ILKAP contains  onePP2C-like domain. ILKAP is widely expressed. Highest levels expressed  in striated muscle. Much lower levels evident in various smooth muscle tissues.  ILKAP may play a role in regulation of cell cycle progression via  dephosphorylation of its substrates whose appropriate phosphorylation states  might be crucial for cell proliferation. ILKAP selectively associates with  integrin linked kinase (ILK), to modulate cell adhesion and growth factor  signaling. ILKAP inhibits the ILK-GSK3B signaling axis and may play an  important role in inhibiting oncogenic transformation. Integrin-linked kinase (  ILK ) plays key roles in a variety of cell functions, including cell  proliferation, adhesion and migration. Within the cell, ILK localizes to  multiple sites, including the cytoplasm, focal adhesion complexes that mediate  cell adhesion to extracellular substrates, as well as cell-cell junctions in  epidermal keratinocytes. Nuclear ILK can be rapidly exported into the cytoplasm  through a CRM1-dependent pathway, and its export is enhanced by the type 2C  protein phosphatase ILKAP. Nuclear localization of ILK in epidermal  keratinocytes is associated with increased DNA synthesis, which is sensitive to  inhibition by ILKAP.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eConcentration\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eActivity\u003c\/b\u003e: Not validated for activity\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSequence\u003c\/b\u003e: Met 1-His 392\u003c\/p\u003e\u003cp\u003e\u003cb\u003ePurity\u003c\/b\u003e:  \u0026gt; 92 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormulation\u003c\/b\u003e: Lyophilized from sterile PBS, pH 7.4\u003cbr\u003eNormally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.\u003cbr\u003ePlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReconstitution\u003c\/b\u003e: Please refer to the printed manual for detailed information.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eEndotoxin\u003c\/b\u003e:  \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCalculated MW\u003c\/b\u003e: 44.3 kDa\u003c\/p\u003e\u003cp\u003e\u003cb\u003eObservedMW\u003c\/b\u003e: 46 kDa\u003c\/p\u003e","brand":"GeneBio Systems","offers":[{"title":"Default Title","offer_id":47972847943780,"sku":"PKSH030793","price":1368.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/no_image_default_image-jpeg_b57497e0-a6ef-4cbb-a4fe-f16a321fcc7a.jpg?v=1772200222","url":"https:\/\/www.genebiosystems.com\/products\/recombinant-human-ilkap-protein-his-tag","provider":"GeneBio ","version":"1.0","type":"link"}