Size: 200ug. Other sizes are also available. Please Inquire.
In Stock: No
Lead time: 10-20 working days
Research Topic: Signal Transduction
Uniprot ID: Q8N6U8
Gene Names: GPR161
Organism: Homo sapiens (Human)
AA Sequence: MSLNSSLSCRKELSNLTEEEGGEGGVII
Expression Region: 1-28aa
Sequence Info: Partial
Source: E.coli
Tag Info: N-terminal GST-tagged
MW: 30 kDa
Alternative Name(s): G-protein coupled receptor RE2
Relevance: Key negative regulator of Shh signaling, which promotes the processing of GLI3 into GLI3R during neural tube development. Recruited by TULP3 and the IFT-A complex to primary cilia and acts as a regulator of the PKA-dependent basal repression machinery in Shh signaling by increasing cAMP levels, leading to promote the PKA-dependent processing of GLI3 into GLI3R and repress the Shh signaling. In presence of SHH, it is roved from primary cilia and is internalized into recycling endosomes, preventing its activity and allowing activation of the Shh signaling. Its ligand is unknown .
Reference: Isolation of cDNA coding for G-protein coupled receptor RE2.Warren C.N., Aronstam R.S., Sharma S.V. Identification of a novel G-protein coupled receptor expressed in distinct brain regions and a defined olfactory zone.Raming K., Konzelmann S., Breer H.Recept. Channels 6:141-151(1998)
Purity: Greater than 90% as determined by SDS-PAGE.
Storage Buffer: Tris-based buffer，50% glycerol
Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.