{"product_id":"recombinant-human-fgf-19-protein","title":"Recombinant Human FGF-19 protein","description":"\u003cp\u003e\u003cb\u003eSize\u003c\/b\u003e:5 ug\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBackground\u003c\/b\u003e:Human FGF-19 is encoded by the FGF19 gene. FGF-19 belongs to the FGF-19 subfamily which has three members FGF-19, 21, 23. FGFs are classically considered to be paracrine factors and are known for their roles in tissue patterning and organogenesis during embryogenesis. By contrast, the FGF-19 subfamily has recently been shown to function in an endocrine manner. Members of this subfamily have poor ability of binding to heparin binding site which is a crucial factor in ligand-receptor complex formation. β-Klotho has been identified as co-factor required for FGF-19, 21, 23 signaling. It can obviously increase ligand-receptor affinity. Unlike most FGFs which bind to and activate more than one FGF receptor, FGF19 is a specific ligand for FGF R4. In FGF-19 transgenic mice, reducing liver triglycerides, increasing fatty acid oxidation, reducing glucose levels and improving insulin sensitivity can be observed.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eResearch_area\u003c\/b\u003e:Immunology\u003c\/p\u003e\u003cp\u003e\u003cb\u003ePurity\u003c\/b\u003e:\u0026gt; 95 % by SDS-PAGE and HPLC analyses.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSource\u003c\/b\u003e:Escherichia coli.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFull product name\u003c\/b\u003e:Recombinant Human Fibroblast Growth Factor-19\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBiological activity\u003c\/b\u003e:Assay #1: Fully biologically active when compared to standard. The biological activity is measured by its binding ability in a functional ELISA. Immobilized rHuFGF R4 at 5 μg\/ml can bind rHuFGF-19 with a linear range of 3-2 ng\/ml.\nAssay #2: Fully biologically active when compared to standard. The ED5 as determined by a cell proliferation assay using Mouse Balb\/c 3T3 cells is less than 15 ng\/ml, corresponding to a specific activity of \u0026gt; 6.7 × 13 IU\/mg.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSynonyms\u003c\/b\u003e:FGF-19, FGF19,Human\u003c\/p\u003e\u003cp\u003e\u003cb\u003eEndotoxin\u003c\/b\u003e:Less than 1 EU\/μg of rHuFGF-19 as determined by LAL method.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage\u003c\/b\u003e:-20ºC\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSequence\u003c\/b\u003e:MRPLAFSDAG PHVHYGWGDP IRLRHLYTSG PHGLSSCFLR IRADGVVDCA RGQSAHSLLE IKAVALRTVA IKGVHSVRYL CMGADGKMQG LLQYSEEDCA FEEEIRPDGY NVYRSEKHRL PVSLSSAKQR QLYKNRGFLP LSHFLPMLPM VPEEPEDLRG HLESDMFSSP LETDSMDPFG LVTGLEAVRS PSFEK\u003c\/p\u003e\u003cp\u003e\u003cb\u003euniprot accession\u003c\/b\u003e:O9575\u003c\/p\u003e\u003cp\u003e\u003cb\u003eMolecular weight\u003c\/b\u003e:Approximately 21.8 kDa, a single non-glycosylated polypeptide chain containing 195 amino acids.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormulation\u003c\/b\u003e:\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReconstitution\u003c\/b\u003e:Lyophilized from a .2 μm filtered concentrated solution in PBS, pH 7.4.\u003c\/p\u003e","brand":"GeneBio Systems","offers":[{"title":"Default Title","offer_id":48654020116580,"sku":"CD01806","price":473.09,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/no_image_default_image-jpeg_5c954ad7-398a-40f6-8775-6078310edccd.jpg?v=1782767902","url":"https:\/\/www.genebiosystems.com\/products\/recombinant-human-fgf-19-protein","provider":"GeneBio ","version":"1.0","type":"link"}