{"product_id":"recombinant-human-dapk3-zipk-protein-gst-tag","title":"Recombinant Human DAPK3\/ZIPK Protein (GST Tag)","description":"\u003cp\u003e\u003cb\u003eSize\u003c\/b\u003e: 50μg\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage\u003c\/b\u003e: Store at \u0026lt; -20°C, stable for 6 months. Please minimize freeze-thaw cycles.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eShipping\u003c\/b\u003e: This product is provided as liquid. It is shipped at frozen temperature with blue ice\/gel packs. Upon receipt, store it immediately at \u0026lt; - 20°C.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eExp date\u003c\/b\u003e: 12 months\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCategory ID_II\u003c\/b\u003e: Recombinant Proteins\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCategory ID_III\u003c\/b\u003e: Others\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAbbreviation\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eTarget Synonym\u003c\/b\u003e: DLK;ZIP;ZIPK\u003c\/p\u003e\u003cp\u003e\u003cb\u003eResearch Areas\u003c\/b\u003e: Signal Transduction;Cell biology;Cancer;\u003c\/p\u003e\u003cp\u003e\u003cb\u003eConjugation\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eTarget Species\u003c\/b\u003e: Human\u003c\/p\u003e\u003cp\u003e\u003cb\u003eExpression Host\u003c\/b\u003e: Baculovirus-Insect Cells\u003c\/p\u003e\u003cp\u003e\u003cb\u003eApplication\u003c\/b\u003e: Enzyme\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFusion tag\u003c\/b\u003e: N-GST\u003c\/p\u003e\u003cp\u003e\u003cb\u003eUNIProt ID\u003c\/b\u003e: O43293\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAccession\u003c\/b\u003e: NP_001339.1\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBackground\u003c\/b\u003e: Death-associated protein kinase 3,  also known as DAP kinase 3, ZIP-kinase, DAPK3 and ZIPK, is a nucleus and  cytoplasm protein which belongs to theprotein kinase superfamily, CAMK  Ser\/Thr protein kinase family and DAP kinase subfamily. DAPK3 \/ ZIPK contains  oneprotein kinase domain. It is a serine\/threonine kinase which acts as a  positive regulator of apoptosis. It phosphorylates histone H3 on 'Thr-11' at  centromeres during mitosis. DAPK3 \/ ZIPK is a homodimer or forms heterodimers  with ATF4. Both interactions require an intact leucine zipper domain and  oligomerization is required for full enzymatic activity. It also binds to DAXX  and PAWR, possibly in a ternary complex which plays a role in caspase  activation. DAPK3 \/ ZIPK regulates myosin light chain phosphatase through  phosphorylation of MYPT1 thereby regulating the assembly of the actin  cytoskeleton, cell migration, invasiveness of tumor cells, smooth muscle  contraction and neurite outgrowth. It is involved in the formation of promyelocytic  leukemia protein nuclear body (PML-NB), one of many subnuclear domains in the  eukaryotic cell nucleus, and which is involved in oncogenesis and viral  infection.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eConcentration\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eActivity\u003c\/b\u003e: The specific activity was determined to be 5 nmol\/min\/mg using MBP as substrate. \u003c\/p\u003e\u003cp\u003e\u003cb\u003eSequence\u003c\/b\u003e: Met 1-Arg 454\u003c\/p\u003e\u003cp\u003e\u003cb\u003ePurity\u003c\/b\u003e:  \u0026gt; 85 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormulation\u003c\/b\u003e: Supplied as sterile solution of 20mM Tris, 500mM NaCl, 10mM GSH, pH 7.4\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReconstitution\u003c\/b\u003e: Not Applicable\u003c\/p\u003e\u003cp\u003e\u003cb\u003eEndotoxin\u003c\/b\u003e:  \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCalculated MW\u003c\/b\u003e: 79.0 kDa\u003c\/p\u003e\u003cp\u003e\u003cb\u003eObservedMW\u003c\/b\u003e: 70 kDa\u003c\/p\u003e","brand":"GeneBio Systems","offers":[{"title":"Default Title","offer_id":47972820975716,"sku":"PKSH030384","price":1044.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/no_image_default_image-jpeg_2cebb6dc-91d5-4c19-b95e-3e83dfb4b218.jpg?v=1772198051","url":"https:\/\/www.genebiosystems.com\/products\/recombinant-human-dapk3-zipk-protein-gst-tag","provider":"GeneBio ","version":"1.0","type":"link"}