{"product_id":"recombinant-human-dapk1-dap-kinase-1-protein-aa-1-363-his-gst-tag","title":"Recombinant Human DAPK1\/DAP Kinase 1 Protein (aa 1-363, His \u0026 GST Tag)","description":"\u003cp\u003e\u003cb\u003eSize\u003c\/b\u003e: 50μg\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage\u003c\/b\u003e: Store at \u0026lt; -20°C, stable for 6 months. Please minimize freeze-thaw cycles.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eShipping\u003c\/b\u003e: This product is provided as liquid. It is shipped at frozen temperature with blue ice\/gel packs. Upon receipt, store it immediately at \u0026lt; - 20°C.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eExp date\u003c\/b\u003e: 12 months\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCategory ID_II\u003c\/b\u003e: Recombinant Proteins\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCategory ID_III\u003c\/b\u003e: Others\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAbbreviation\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eTarget Synonym\u003c\/b\u003e: DAPK\u003c\/p\u003e\u003cp\u003e\u003cb\u003eResearch Areas\u003c\/b\u003e: Cell Biology,Signal Transduction,Cancer\u003c\/p\u003e\u003cp\u003e\u003cb\u003eConjugation\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eTarget Species\u003c\/b\u003e: Human\u003c\/p\u003e\u003cp\u003e\u003cb\u003eExpression Host\u003c\/b\u003e: Baculovirus-Insect Cells\u003c\/p\u003e\u003cp\u003e\u003cb\u003eApplication\u003c\/b\u003e: Enzyme\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFusion tag\u003c\/b\u003e: N-His-GST\u003c\/p\u003e\u003cp\u003e\u003cb\u003eUNIProt ID\u003c\/b\u003e: P53355\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAccession\u003c\/b\u003e: P53355-1\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBackground\u003c\/b\u003e: Death-associated protein kinase 1,  also known as DAP kinase 1, DAPK1 and DAPK, is a cytoplasm protein which  belongs to theprotein kinase superfamily, CAMK Ser \/ Thr protein kinase  family and DAP kinase subfamily. DAPK1 contains tenANK repeats,  onedeath domain and oneprotein kinase domain. DAPK1 is a calcium \/  calmodulin-dependent serine\/threonine kinase which acts as a positive regulator  of apoptosis. DAPK1 gene is a candidate tumor suppressor (TSG) and the abnormal  methylation of DAPK1 gene has been found in many carcinomas. DAPK1  over-expression can induce cell apoptosis and inhibit tumor cell metastasis.  DAPK1 gene over-expression could suppress PGCl3 cells malignant phenotype,  inhibit PGCl3 cells growth, invasive, migration and adhesion ability,  upregulate p53 gene and downregulate bcl-2 gene. Loss of activity of  death-associated protein kinase 1 ( DAPK1 ) may be an independent factor  affecting survival of non-small cell lung cancer patients. DAPK1 promoter  methylation might play a significant role in the progression of chronic myeloid  leukemia ( CML ).\u003c\/p\u003e\u003cp\u003e\u003cb\u003eConcentration\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eActivity\u003c\/b\u003e: The specific activity was determined to be 20 nmol\/min\/mg using synthetic R11-S6-Peptide (R11-IAKRRRLSSLRASTSKSESSQK) as substrate. \u003c\/p\u003e\u003cp\u003e\u003cb\u003eSequence\u003c\/b\u003e: Met 1-Leu 363\u003c\/p\u003e\u003cp\u003e\u003cb\u003ePurity\u003c\/b\u003e:  \u0026gt; 80 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormulation\u003c\/b\u003e: Supplied as sterile solution of 20mM Tris, 500mM NaCl, pH 8.0, 10% glycerol\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReconstitution\u003c\/b\u003e: Not Applicable\u003c\/p\u003e\u003cp\u003e\u003cb\u003eEndotoxin\u003c\/b\u003e:  \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCalculated MW\u003c\/b\u003e: 69.4 kDa\u003c\/p\u003e\u003cp\u003e\u003cb\u003eObservedMW\u003c\/b\u003e: 64 kDa\u003c\/p\u003e","brand":"GeneBio Systems","offers":[{"title":"Default Title","offer_id":47972817895524,"sku":"PKSH030343","price":1044.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/no_image_default_image-jpeg_a4e0e873-a959-4d2f-b7e5-6e98fdc2b49a.jpg?v=1772197805","url":"https:\/\/www.genebiosystems.com\/products\/recombinant-human-dapk1-dap-kinase-1-protein-aa-1-363-his-gst-tag","provider":"GeneBio ","version":"1.0","type":"link"}