{"product_id":"recombinant-human-csnk1g1-cki-gamma-1-protein-his-gst-tag","title":"Recombinant Human CSNK1G1\/CKI-gamma 1 Protein (His \u0026 GST Tag)","description":"\u003cp\u003e\u003cb\u003eSize\u003c\/b\u003e: 50μg\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage\u003c\/b\u003e: Store at \u0026lt; -20°C, stable for 6 months. Please minimize freeze-thaw cycles.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eShipping\u003c\/b\u003e: This product is provided as liquid. It is shipped at frozen temperature with blue ice\/gel packs. Upon receipt, store it immediately at \u0026lt; - 20°C.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eExp date\u003c\/b\u003e: 12 months\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCategory ID_II\u003c\/b\u003e: Recombinant Proteins\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCategory ID_III\u003c\/b\u003e: Others\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAbbreviation\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eTarget Synonym\u003c\/b\u003e: CK1gamma1\u003c\/p\u003e\u003cp\u003e\u003cb\u003eResearch Areas\u003c\/b\u003e: Signal Transduction;Stem cells\u003c\/p\u003e\u003cp\u003e\u003cb\u003eConjugation\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eTarget Species\u003c\/b\u003e: Human\u003c\/p\u003e\u003cp\u003e\u003cb\u003eExpression Host\u003c\/b\u003e: Baculovirus-Insect Cells\u003c\/p\u003e\u003cp\u003e\u003cb\u003eApplication\u003c\/b\u003e: Enzyme\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFusion tag\u003c\/b\u003e: N-His-GST\u003c\/p\u003e\u003cp\u003e\u003cb\u003eUNIProt ID\u003c\/b\u003e: Q9HCP0\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAccession\u003c\/b\u003e: NP_071331.2\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBackground\u003c\/b\u003e: Casein  kinase I isoform gamma-1, also known as CSNK1G1, is a member of the protein  kinase superfamily, CK1 Ser\/Thr protein kinase family and casein kinase I  subfamily. Thecasein kinase I family of protein kinases are serine \/  threonine-selective enzymes that function as regulators ofsignal  transductionpathways in most eukaryotic cell types. Casein has been used  as a substrate since the earliest days of research on protein phosphorylation.  Casein kinase activity associated with the endoplasmic reticulum of mammary  glands was first characterized in 1974 and its activity was shown to not depend  on cyclic AMP. The CKI family of monomeric serine–threonine protein kinases is  found in eukaryotic organisms from yeast to human. Mammals have seven family  members: alpha, beta 1, gamma 1, gamma 2, gamma 3, delta, and epsilon. The  family members have the highest homology in their kinase domains (53%–98%  identical) and differ from most other protein kinases by the presence of the  sequence S-I-N instead of A-P-E in kinase domain VIII. The CKI family members  appear to have similar substrate specificity and substrate selection is thought  to be regulated via subcellular localization and docking sites in specific  substrates.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eConcentration\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eActivity\u003c\/b\u003e: The specific activity was determined to be 4 nmol\/min\/mg using casein as substrate. \u003c\/p\u003e\u003cp\u003e\u003cb\u003eSequence\u003c\/b\u003e: Met 1-Lys 422\u003c\/p\u003e\u003cp\u003e\u003cb\u003ePurity\u003c\/b\u003e:  \u0026gt; 94 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormulation\u003c\/b\u003e: Supplied as sterile solution of 20mM Tris, 500mM NaCl, 10% glycerol, pH 7.4\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReconstitution\u003c\/b\u003e: Not Applicable\u003c\/p\u003e\u003cp\u003e\u003cb\u003eEndotoxin\u003c\/b\u003e:  \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCalculated MW\u003c\/b\u003e: 76.3 kDa\u003c\/p\u003e\u003cp\u003e\u003cb\u003eObservedMW\u003c\/b\u003e: 66 kDa\u003c\/p\u003e","brand":"GeneBio Systems","offers":[{"title":"Default Title","offer_id":47972819763300,"sku":"PKSH030361","price":1044.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/no_image_default_image-jpeg_f9d33200-028a-41d8-8962-5e6bb2aa0d7c.jpg?v=1772197912","url":"https:\/\/www.genebiosystems.com\/products\/recombinant-human-csnk1g1-cki-gamma-1-protein-his-gst-tag","provider":"GeneBio ","version":"1.0","type":"link"}