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Updated Date: Stock Protein updated on 20171018
Research areas: Others
Target / Protein: URA5
Biologically active: Not Tested
Expression system: Yeast
Species of origin: Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565) (Filobasidiella neoformans)
Delivery time: 3-7 business days
Uniprot ID: P0CS95
AA Sequence: MSSQALDSAKVAFIEAAIEHGVLLFGNFTLKSGRQSPYFFNAGLLYSSSLLSTTAQAYAKVLSSSRIPDFDVLFGPAYKGISLAAVSAVSLYQQTGKDIGYCYNRKEKKDHGEGGTMVGAPLKGRIVIIDDVLTSGKAIREAIDILKASPEAKLVGIVQLVDRQEKGQSGSGKSTVQEVEEEFGVPVEPIIGLDDIVKYLESSGKWEKELQEVRKYRAEYGVQRS
Tag info: N-terminal 10xHis-tagged and C-terminal Myc-tagged
Expression Region: 1-225aa
Protein length: Full Length
MW: 28.4 kDa
Relevance: Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP).
Reference: "Isolation of the URA5 gene from Cryptococcus neoformans var. neoformans and its use as a selective marker for transformation." Edman J.C., Kwon-Chung K.J. Mol. Cell. Biol. 10:4538-4544(1990)
Purity: Greater than 90% as determined by SDS-PAGE.
Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.