{"product_id":"recombinant-2019-ncov-papain-like-protease","title":"Recombinant 2019-nCoV Papain-Like Protease","description":"\u003cp\u003e\u003cb\u003eSize\u003c\/b\u003e:50ug\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDescription\u003c\/b\u003e:Recombinant 2019-nCoV Papain-like Protease is produced by our E.coli expression system and the target gene encoding Glu1564-Lys1878 is expressed.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAccession\u003c\/b\u003e:QHD43415.1\u003c\/p\u003e\u003cp\u003e\u003cb\u003eMolecular weight\u003c\/b\u003e:35.8 KDa\u003c\/p\u003e\u003cp\u003e\u003cb\u003eApparent molecular weight\u003c\/b\u003e:34 KDa, reducing conditions\u003c\/p\u003e\u003cp\u003e\u003cb\u003eOther names\u003c\/b\u003e:Papain-like Protease; PLpro; PL-PRO; pp1a; Replicase polyprotein 1a\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage condition\u003c\/b\u003e:Lyophilized protein should be stored at \u0026lt; -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20°C for 3 months.\u003c\/p\u003e\u003cp\u003e\u003cb\u003ePurity\u003c\/b\u003e:Greater than 95% as determined by reducing SDS-PAGE.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eEndotoxin\u003c\/b\u003e:\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBiological activity\u003c\/b\u003e:Measured by its ability to convert the fluorogenic peptide substrate, Arg-Leu-Arg-Gly-Gly-AMC (RLRGGAMC) to RLRGG and 7-amino-4-methylcoumarin (AMC). The Specific Activity is 241.7 pmol\/min\/μg.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eRedissolve\u003c\/b\u003e:\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage\u003c\/b\u003e:Store at ≤-70°C, stable for 6 months after receipt.\nStore at ≤-70°C, stable for 3 months under sterile conditions after opening. \nPlease minimize freeze-thaw cycles.      \u003c\/p\u003e\u003cp\u003e\u003cb\u003eDelivery condition\u003c\/b\u003e:The product is shipped on dry ice\/polar packs.\nUpon receipt, store it immediately at the temperature listed below.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBackground\u003c\/b\u003e:Replication of severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) requires proteolytic processing of the replicase polyprotein by two viral cysteine proteases, a chymotrypsin-like protease (3CLpro) and a papain-like protease (PLpro). These proteases are important targets for development of antiviral drugs that would inhibit viral replication and reduce mortality associated with outbreaks of SARS-CoV. PLpro is a cysteine protease located within the non-structural protein 3 (NS3) section of the viral polypeptide. PLPro activity is required to process the viral polyprotein into functional, mature subunits; specifically, PLPro cleaves a site at the amino-terminus of the viral replicase region.  In addition to its role in viral protein maturation, PLPro possesses a deubiquitinating and deISGylating activity.  In vivo, this protease antagonizes innate immunity by inhibiting IRF3-induced production of type I interferons.\u003c\/p\u003e","brand":"GeneBio Systems","offers":[{"title":"Default Title","offer_id":48647905738852,"sku":"EPT114","price":1879.36,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/no_image_default_image-jpeg_d5f18c6b-cb44-4857-a5df-f15916874c7a.jpg?v=1782704501","url":"https:\/\/www.genebiosystems.com\/products\/recombinant-2019-ncov-papain-like-protease","provider":"GeneBio ","version":"1.0","type":"link"}