{"product_id":"rat-tyrosine-kinase-with-immunoglobulin-like-and-egf-like-domains-2-tie-2-elisa-kit","title":"Rat Tyrosine kinase with immunoglobulin-like and EGF-like domains 2 (Tie-2) ELISA Kit","description":"\u003cp\u003e\u003cb\u003eSize\u003c\/b\u003e: 96T. Other sizes are also available. \u003c\/p\u003e\u003cp\u003e\u003cb\u003eSpecies Reactivity\u003c\/b\u003e: Rat (Rattus norvegicus)\u003c\/p\u003e\u003cp\u003e\u003cb\u003eUniProt\u003c\/b\u003e: N\/A\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAbbreviation\u003c\/b\u003e: Tie-2\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAlternative Names\u003c\/b\u003e: N\/A\u003c\/p\u003e\u003cp\u003e\u003cb\u003eApplication\u003c\/b\u003e: ELISA\u003c\/p\u003e\u003cp\u003e\u003cb\u003eRange\u003c\/b\u003e: 0.312-20 ng\/mL\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSensitivity\u003c\/b\u003e: 0.135 ng\/mL\u003c\/p\u003e\u003cp\u003e\u003cb\u003eTest principle\u003c\/b\u003e: This assay employs a two-site sandwich ELISA to quantitate Tie-2 in samples. An antibody specific for Tie-2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTie-2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for Tie-2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Tie-2 bound in the initial step. The color development is stopped and the intensity of the color is measured.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Overview\u003c\/b\u003e: Tie-2 is a receptor tyrosine kinase that is expressed primarily on endothelial cells (ECs) and plays a critical role in vascular development. The Tie-2 gene encodes a protein of 1122 amino acids. The extracellular region has three distinct structural motifs including two immunoglobulin (Ig)-like loops separated by three EGF-like repeats, and three repeats with fibronectin type III homology located after the second Ig loop. The intracellular portion of Tie-2 contains two tyrosine kinase domains that, when phosphorylated, interact with a number of binding partners including Grb2, Grb7, Grb14, Shp2, the p85 subunit of phosphatidylinositol 3-kinase (PI3K), and Dok-R. Deletion of the last 16 amino acids of the intracellular C-terminus results in increased levels of autophosphorylation, suggesting that it may play an autoinhibitory role.\n\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStability\u003c\/b\u003e: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. \nThe loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).\u003c\/p\u003e","brand":"GeneBio Systems","offers":[{"title":"Default Title","offer_id":40500656537700,"sku":"AE59663RA","price":1156.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/no_image_default_image-jpeg_a00183f4-3c16-48ed-9c9a-f3c7e5b3b233.jpg?v=1660528693","url":"https:\/\/www.genebiosystems.com\/products\/rat-tyrosine-kinase-with-immunoglobulin-like-and-egf-like-domains-2-tie-2-elisa-kit","provider":"GeneBio ","version":"1.0","type":"link"}