{"product_id":"rat-allopregnanolone-3-5-tetrahydroprogesterone-ap-3-5-thp-elisa-kit","title":"Rat Allopregnanolone\/3?,5?-tetrahydroprogesterone (AP\/3?-5?-THP) ELISA Kit","description":"\u003cp\u003e\u003cb\u003eSpecies Reactivity\u003c\/b\u003e:Rat (Rattus norvegicus)\u003c\/p\u003e\u003cp\u003e\u003cb\u003eUniProt\u003c\/b\u003e:N\/A\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAbbreviation\u003c\/b\u003e:AP\/3?-5?-THP\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAlternative Names\u003c\/b\u003e:N\/A\u003c\/p\u003e\u003cp\u003e\u003cb\u003eApplication\u003c\/b\u003e:ELISA\u003c\/p\u003e\u003cp\u003e\u003cb\u003eRange\u003c\/b\u003e:1.56-100 ng\/mL\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSensitivity\u003c\/b\u003e:0.78 ng\/mL\u003c\/p\u003e\u003cp\u003e\u003cb\u003eIntra-AssayCV\u003c\/b\u003e:?3.9%\u003c\/p\u003e\u003cp\u003e\u003cb\u003eInter-AssayCV\u003c\/b\u003e:?10.8%\u003c\/p\u003e\u003cp\u003e\u003cb\u003eRecovery\u003c\/b\u003e:1.01\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSample Type\u003c\/b\u003e:Serum, Plasma, Other biological fluids\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDetection Method\u003c\/b\u003e:Sandwich\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAnalysis Method??\u003c\/b\u003e:Quantitive\u003c\/p\u003e\u003cp\u003e\u003cb\u003eTest principle\u003c\/b\u003e:This assay employs a two-site sandwich ELISA to quantitate AP\/3?-5?-THP in samples. An antibody specific for AP\/3?-5?-THP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyAP\/3?-5?-THP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AP\/3?-5?-THP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AP\/3?-5?-THP bound in the initial step. The color development is stopped and the intensity of the color is measured.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Overview\u003c\/b\u003e:\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStability\u003c\/b\u003e:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. \nThe loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"Default Title","offer_id":40500642873444,"sku":"AE56223RA","price":1600.99,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/no_image_default_image-jpeg_af4878bb-cf29-44ae-a24f-586cf01473ec.jpg?v=1660528297","url":"https:\/\/www.genebiosystems.com\/products\/rat-allopregnanolone-3-5-tetrahydroprogesterone-ap-3-5-thp-elisa-kit","provider":"GeneBio ","version":"1.0","type":"link"}