{"product_id":"purified-rabbit-anti-nt5c3-antibody","title":"Purified Rabbit  anti-NT5C3 Antibody","description":"\u003cp\u003e\u003cb\u003eSize 1\u003c\/b\u003e: 100 ul\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSynonyms \u003c\/b\u003e: NT5C3; P5N1; UMPH1; Cytosolic 5'-nucleotidase 3A; Cytosolic 5'-nucleotidase 3; Cytosolic 5'-nucleotidase III; cN-III; Pyrimidine 5'-nucleotidase 1; P5'N-1; P5N-1; PN-I; Uridine 5'-monophosphate hydrolase 1; p36\u003c\/p\u003e\u003cp\u003e\u003cb\u003ehost\u003c\/b\u003e: Rabbit\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）\u003c\/b\u003e: H\u003c\/p\u003e\u003cp\u003e\u003cb\u003eApplications\u003c\/b\u003e: WB, IH, IF\/IC\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBackground\u003c\/b\u003e: Rabbit polyclonal antibody to NT5C3\u003c\/p\u003e\u003cp\u003e\u003cb\u003eConcentration\u003c\/b\u003e: 1mg\/mL\u003c\/p\u003e\u003cp\u003e\u003cb\u003eImmunogen\u003c\/b\u003e: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human NT5C3. The exact sequence is proprietary.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eWB description\u003c\/b\u003e: Western blot analysis of NT5C3 expression in K562 (A), Hela (B) whole cell lysates.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eIHC description\u003c\/b\u003e: Immunohistochemical analysis of NT5C3 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eIF\/ICC description\u003c\/b\u003e: Immunofluorescent analysis of NT5C3 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eIP description\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eFC description\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eChIP description\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003ePurification_Method\u003c\/b\u003e: The antibody was purified by immunogen affinity chromatography.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eClonality\u003c\/b\u003e: Polyclonal\u003c\/p\u003e\u003cp\u003e\u003cb\u003eConjugation\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eBuffer\u003c\/b\u003e: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDilution\u003c\/b\u003e: WB (1\/500 - 1\/1000), IH (1\/50 - 1\/200), IF\/IC (1\/50 - 1\/200)\u003c\/p\u003e\u003cp\u003e\u003cb\u003eGene ID(Human)\u003c\/b\u003e: 51251\u003c\/p\u003e\u003cp\u003e\u003cb\u003eGene ID (Mouse)\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eGene ID(Rat)\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eSwissProt (Human)\u003c\/b\u003e: Q9H0P0\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSwissProt (Mouse)\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eSwissProt (Rat)\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage\u003c\/b\u003e: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze\/thaw cycles.\u003c\/p\u003e","brand":"GeneBio Systems","offers":[{"title":"Default Title","offer_id":48738950807652,"sku":"DL99264A","price":837.2,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/no_image_default_image-jpeg_295ec87c-6677-42fb-af0b-58569d309b6c.jpg?v=1783816511","url":"https:\/\/www.genebiosystems.com\/products\/purified-rabbit-anti-nt5c3-antibody","provider":"GeneBio ","version":"1.0","type":"link"}