{"product_id":"phospho-wee1-ser642-antibody-cat-csb-pa062918","title":"Phospho-WEE1 (Ser642) Antibody - Cat. #: CSB-PA062918","description":"\u003cp\u003e\u003cb\u003eSize \u003c\/b\u003e:100ul\u003c\/p\u003e\u003cp\u003e\u003cb\u003eClone Number\u003c\/b\u003e:\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAliases\u003c\/b\u003e:DKFZp686I18166 antibody; EC 2.7.10.2 antibody; FLJ16446 antibody; MGC105683 antibody; OTTHUMP00000231338 antibody; OTTHUMP00000231339 antibody; Wee 1 antibody; WEE 1 homolog 1 (S. pombe) antibody; WEE1 antibody; WEE1 homolog (S. pombe) antibody; Wee1 homolog antibody; WEE1 homolog S. pombe antibody; Wee1 like protein kinase antibody; Wee1 tyrosine kinase antibody; Wee1+ homolog antibody; Wee1+ S. pombe homolog antibody; WEE1, S. pombe, homolog of antibody; WEE1, somatic antibody; Wee1-like protein kinase antibody; WEE1_HUMAN antibody; WEE1A antibody; Wee1A kinase antibody; WEE1hu antibody\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Type\u003c\/b\u003e:Polyclonal Antibody\u003c\/p\u003e\u003cp\u003e\u003cb\u003eImmunogen Species\u003c\/b\u003e:Homo sapiens (Human)\u003c\/p\u003e\u003cp\u003e\u003cb\u003eUniProt ID\u003c\/b\u003e:P30291\u003c\/p\u003e\u003cp\u003e\u003cb\u003eImmunogen\u003c\/b\u003e:Peptide sequence around phosphorylation site of Serine 642(S-V-S(p)-L-T) derived from Human WEE1.\n\u003c\/p\u003e\u003cp\u003e\u003cb\u003eRaised in\u003c\/b\u003e:Rabbit\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSpecies Reactivity\u003c\/b\u003e:Human, Mouse, Rat\u003c\/p\u003e\u003cp\u003e\u003cb\u003eTested Applications\u003c\/b\u003e:ELISA, WB; WB:1:500-1:1000\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBackground\u003c\/b\u003e:\u003c\/p\u003e\u003cp\u003eActs as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M\/G1 phase, probably due to its degradation.\u003cbr\u003e\u003cbr\u003eWatanabe N., EMBO J. 14:1878-1891(1995).\u003cbr\u003eCichutek A., Cytogenet. Cell Genet. 93:277-283(2001).\u003cbr\u003eIgarashi M., Nature 353:80-83(1991)\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eClonality\u003c\/b\u003e:Polyclonal\u003c\/p\u003e\u003cp\u003e\u003cb\u003eIsotype\u003c\/b\u003e:IgG\u003c\/p\u003e\u003cp\u003e\u003cb\u003ePurification Method\u003c\/b\u003e:Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi\u003c\/p\u003e\u003cp\u003e\u003cb\u003eConjugate\u003c\/b\u003e:Non-conjugated\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBuffer\u003c\/b\u003e:Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eForm\u003c\/b\u003e:liquid\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStroage\u003c\/b\u003e:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eTarget Names\u003c\/b\u003e:WEE1\u003c\/p\u003e\u003cp\u003e\u003cb\u003eResearch Areas\u003c\/b\u003e:Epigenetics and Nuclear Signaling;Cell biology\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"Default Title","offer_id":40484029694052,"sku":"CSB-PA062918","price":1051.32,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/no_image_default_image-jpeg_70e3cc60-ed34-4ef1-b016-17d11e89c3c5.jpg?v=1660086439","url":"https:\/\/www.genebiosystems.com\/products\/phospho-wee1-ser642-antibody-cat-csb-pa062918","provider":"GeneBio ","version":"1.0","type":"link"}