{"product_id":"phospho-pkc-th-y90-rabbit-pab-antibody","title":"Phospho PKC θ (Y90) rabbit pAb","description":"\u003cp\u003e\u003cb\u003eSize\u003c\/b\u003e: 100μL\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSource\u003c\/b\u003e:Rabbit\u003c\/p\u003e\u003cp\u003e\u003cb\u003eApplications\u003c\/b\u003e:WB;IHC\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReactivity\u003c\/b\u003e:Human;Mouse;Rat\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDilution\u003c\/b\u003e:WB 1:500-2000;IHC-p 1:50-300\u003c\/p\u003e\u003cp\u003e\u003cb\u003eImmunogen\u003c\/b\u003e:Synthesized peptide derived from human Phospho PKC θ (Y90)\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage_stability\u003c\/b\u003e:-20°C\/1 year\u003c\/p\u003e\u003cp\u003e\u003cb\u003eClonality\u003c\/b\u003e:Polyclonal\u003c\/p\u003e\u003cp\u003e\u003cb\u003eIsotype\u003c\/b\u003e:IgG\u003c\/p\u003e\u003cp\u003e\u003cb\u003eConcentration\u003c\/b\u003e:1 mg\/ml\u003c\/p\u003e\u003cp\u003e\u003cb\u003eObserved_band(KD)\u003c\/b\u003e:78kD\u003c\/p\u003e\u003cp\u003e\u003cb\u003eHuman_gene_id\u003c\/b\u003e:5588\u003c\/p\u003e\u003cp\u003e\u003cb\u003eHuman_swiss_prot_no\u003c\/b\u003e:Q04759\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSubcellular_location\u003c\/b\u003e:Cytoplasm. Cell membrane; Peripheral membrane protein. In resting T-cells, mostly localized in cytoplasm. In response to TCR stimulation, associates with lipid rafts and then localizes in the immunological synapse.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eOther_name\u003c\/b\u003e:Protein kinase C theta type (EC 2.7.11.13;nPKC-theta)\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBackground\u003c\/b\u003e:catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,domain:The C1 domain, containing the phorbol ester\/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain.,enzyme regulation:Three specific sites; Thr-538 (activation loop of the kinase domain), Ser-676 (turn motif) and Ser-695 (hydrophobic region), need to be phosphorylated for its full activation.,function:PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.,function:This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Essential for T-cell receptor (TCR)-mediated T-cell activation, but is dispensable during TCR-dependent thymocyte development. Links the TCR signaling complex to the activation of NF-kappa-B in mature T lymphocytes. Required for interleukin-2 (IL2) production.,PTM:Autophosphorylation at Thr-219 is required for targeting to the TCR and cellular function of PKC upon antigen receptor ligation.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. AGC Ser\/Thr protein kinase family. PKC subfamily.,similarity:Contains 1 AGC-kinase C-terminal domain.,similarity:Contains 1 C2 domain.,similarity:Contains 1 protein kinase domain.,similarity:Contains 2 phorbol-ester\/DAG-type zinc fingers.,subunit:Interacts with TXNL2\/PICOT.,tissue specificity:Skeletal muscle, megakaryoblastic cells and platelets.,\u003c\/p\u003e","brand":"GeneBio Systems","offers":[{"title":"Default Title","offer_id":48647880409188,"sku":"ES20173","price":477.4,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/no_image_default_image-jpeg_455a83f5-35d2-4a02-9813-4614741273b7.jpg?v=1782704027","url":"https:\/\/www.genebiosystems.com\/products\/phospho-pkc-th-y90-rabbit-pab-antibody","provider":"GeneBio ","version":"1.0","type":"link"}