{"product_id":"nos2-elisa-kit-for-mouse","title":"NOS2 ELISA kit (Mouse)","description":"\u003cp\u003e\u003cb\u003eSize\u003c\/b\u003e: 96Tests\u003c\/p\u003e\u003cp\u003e\u003cb\u003e# of Times Cited in literature\u003c\/b\u003e: 23\u003c\/p\u003e\u003cp\u003e\u003cb\u003ePrepare Time\u003c\/b\u003e: 1-3 days(please inquire for mutiple units)\u003c\/p\u003e\u003cp\u003e\u003cb\u003eTarget Name\u003c\/b\u003e: NOS2\u003c\/p\u003e\u003cp\u003e\u003cb\u003eTarget Full Name\u003c\/b\u003e: Nitric Oxide Synthase 2, Inducible\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAlternative Names\u003c\/b\u003e: NOS2A; INOS; HEP-NOS; I-NOS; Hepatocytes Oxide Synthase; Peptidyl-cysteine S-nitrosylase NOS2\u003c\/p\u003e\u003cp\u003e\u003cb\u003eTarget Species\u003c\/b\u003e: Mouse\u003c\/p\u003e\u003cp\u003e\u003cb\u003eUniprot\u003c\/b\u003e: P29477\u003c\/p\u003e\u003cp\u003e\u003cb\u003eGene ID\u003c\/b\u003e: 18126\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFeatured Series\u003c\/b\u003e: SE kit\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFeatured Series Function\u003c\/b\u003e: Detects protein (regular version)\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSpecificity\u003c\/b\u003e: Reactive with Mouse NOS2 \/ Nitric Oxide Synthase 2, Inducible\u003c\/p\u003e\u003cp\u003e\u003cb\u003eMethod\u003c\/b\u003e: Colormetric\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDetection principle\u003c\/b\u003e: Double-antibody Sandwich\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDetection\nrange\u003c\/b\u003e: 0.312-20ng\/mL\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSensitivity\u003c\/b\u003e: 0.116ng\/mL\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAssay Time\u003c\/b\u003e: 3h\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSample Size\u003c\/b\u003e: 100uL\u003c\/p\u003e\u003cp\u003e\u003cb\u003eRecommended\/Predicted\nSample Types\u003c\/b\u003e: Tissue Homogenates, Cell Lysates, Cell Culture Supernates and other Biological Fluids\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAssay Precision\u003c\/b\u003e: Intra-Assay: CV\u0026lt;10%, Inter-Assay: CV\u0026lt;12%\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReproducibility test menthod\u003c\/b\u003e: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Nitric Oxide Synthase 2, Inducible (NOS2) were tested 20 times on one plate, respectively.\nInter-assay Precision (Precision between assays): 3 samples with low, middle and high level Nitric Oxide Synthase 2, Inducible (NOS2) were tested on 3 different plates, 8 replicates in each plate.\nCV(%) = SD\/meanX100\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage\u003c\/b\u003e: 4°C for 1 month\/ -20°C for long-term(One year within shelf life)\u003c\/p\u003e\u003cp\u003e\u003cb\u003eShelf-life\u003c\/b\u003e: 12 months\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSpecificity\u003c\/b\u003e: This assay has high sensitivity and excellent specificity for detection of Nitric Oxide Synthase 2, Inducible (NOS2).\nNo significant cross-reactivity or interference between Nitric Oxide Synthase 2, Inducible (NOS2) and analogues was observed.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStability\u003c\/b\u003e: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.\nTo minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAssay procedure summary\u003c\/b\u003e: 1. Prepare all reagents, samples and standards;\n2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;\n3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;\n4. Aspirate and wash 3 times;\n5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;\n6. Aspirate and wash 5 times;\n7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;\n8. Add 50µL Stop Solution. Read at 450nm immediately.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eTest principle\u003c\/b\u003e: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nitric Oxide Synthase 2, Inducible (NOS2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nitric Oxide Synthase 2, Inducible (NOS2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nitric Oxide Synthase 2, Inducible (NOS2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Nitric Oxide Synthase 2, Inducible (NOS2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eResearch Area\u003c\/b\u003e: Enzyme \u0026amp; Kinase;Metabolic pathway;Tumor immunity;Cardiovascular biology;\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReferences Citing This Product\u003c\/b\u003e: \u003ca href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/20713153\"\u003eHigh-molecular weight hyaluronan reduced renal PKC activation in genetically diabetic mice\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"http:\/\/www.sciencedirect.com\/science\/article\/pii\/S0925443911001323\"\u003eHyaluronan reduces inflammation in experimental arthritis by modulating TLR-2 and TLR-4 cartilage expression\u003c\/a\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"http:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1742-4658.2012.08598.x\/abstract\"\u003eAdenosine A2a Receptor Activation and Hyaluronan Fragment Inhibition Reduce Inflammation in Mouse Articular Chondrocytes Stimulated with Il-1beta\u003c\/a\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"http:\/\/www.sciencedirect.com\/science\/article\/pii\/S0378111911007347\"\u003eHyaluronan in part mediates IL-1beta-induced inflammation in mouse chondrocytes by up-regulating CD44 receptors\u003c\/a\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"http:\/\/www.plosone.org\/article\/info%3Adoi%2F10.1371%2Fjournal.pone.0055792\"\u003eRegulation of Microglia Activity by Glaucocalyxin-A: Attenuation of Lipopolysaccharide-Stimulated Neuroinflammation through NF-κB and p38 MAPK Signaling Pathways\u003c\/a\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/22234777\"\u003eThe inhibition of hyaluronan degradation reduced pro-inflammatory cytokines in mouse synovial fibroblasts subjected to collagen-induced arthritis.\u003c\/a\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/24013642\"\u003eDecalepis hamiltonii inhibits tumor progression and metastasis by regulating the inflammatory mediators and nuclear factor κB subunits\u003c\/a\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/23567245\"\u003eInhibition of UVB-induced skin phototoxicity by a grape seed extract as modulator of nitrosative stress, ERK\/NF-kB signaling pathway and apoptosis, in SKH-1 mice\u003c\/a\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"http:\/\/www.sciencedirect.com\/science\/article\/pii\/S1567576913004153\"\u003eProtective effect of marine mangrove Rhizophora apiculata on acetic acid induced experimental colitis by regulating anti-oxidant enzymes, inflammatory mediators and nuclear factor-kappa B subunits\u003c\/a\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/25222193\"\u003eUtility of Apc-mutant rats with a colitis-associated colon carcinogenesis model for chemoprevention studies\u003c\/a\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/25673209\"\u003eBeta-arrestin 1 is involved in the catabolic response stimulated by hyaluronan degradation in mouse chondrocytes\u003c\/a\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/26349607\"\u003eProtective Effect of\u003c\/a\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/25738525\"\u003eAcacia ferruginea inhibits inflammation by regulating inflammatory iNOS and COX-2\u003c\/a\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/26972100\"\u003eAnti-oxidative, anti-secretory and anti-inflammatory activities of the extract from the root bark of Lycium chinense (Cortex Lycii) against gastric ulcer in mice\u003c\/a\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/27157496\"\u003eToxoplasma gondii GRA15II effector-induced M1 cells ameliorate liver fibrosis in mice infected with Schistosomiasis japonica\u003c\/a\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"http:\/\/www.tandfonline.com\/doi\/abs\/10.1080\/09540105.2017.1306494\"\u003eInfluences of Hyriopsis cumingii polysaccharides on mice immunosignaling molecules and T lymphocyte differentiation\u003c\/a\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/26972100\"\u003eAnti-oxidative, anti-secretory and anti-inflammatory activities of the extract from the root bark ofLycium chinense (Cortex Lycii) against gastric ulcer in mice.\u003c\/a\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/27435276\"\u003eProtective Effect of Zingiber officinale Against Dalton's Lymphoma Ascites Tumour byRegulating Inflammatory Mediator and Cytokines.\u003c\/a\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"http:\/\/pubs.rsc.org\/-\/content\/articlehtml\/2017\/ra\/c7ra08879e\"\u003eAnti-ulcerogenic effect of KFP-H008 against ethanol-induced gastric ulcer via p38 MAPK\/NF-κB pathway\u003c\/a\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/29547569\"\u003eMelatonin balance the autophagy and apoptosis by regulating UCP2 in the LPS-induced cardiomyopathy\u003c\/a\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/29678767\"\u003eTargeted inhibition of tumor survival, metastasis and angiogenesis by Acacia ferruginea mediated regulation of VEGF, inflammatory mediators, cytokine profile and …\u003c\/a\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"https:\/\/journals.plos.org\/plosone\/article?id=10.1371\/journal.pone.0208279\"\u003eMacrophage expression of E3 ubiquitin ligase Grail protects mice from lipopolysaccharide-induced hyperinflammation and organ injury\u003c\/a\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/34684662\/\"\u003eErinacine A-Enriched Hericium erinaceus Mycelium Delays Progression of Age-Related Cognitive Decline in Senescence Accelerated Mouse Prone 8 (SAMP8) Mice\u003c\/a\u003e\u003cp\u003e \u003c\/p\u003e","brand":"GeneBio Systems","offers":[{"title":"Default Title","offer_id":48696703058020,"sku":"SEA837Mu","price":1360.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/no_image_default_image-jpeg_6ea08570-7193-4dd9-b31b-55634fa7510a.jpg?v=1783134693","url":"https:\/\/www.genebiosystems.com\/products\/nos2-elisa-kit-for-mouse","provider":"GeneBio ","version":"1.0","type":"link"}