{"product_id":"mixsure-2-perfectstart-r-green-qpcr-supermix","title":"MixSure™ 2×PerfectStart®  Green qPCR SuperMix","description":"\u003cdiv class=\"page-title anchor\" data-anchor=\"introduce\"\u003e\n\u003ch3\u003eProduct Details\u003c\/h3\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"product-brief\"\u003e\n\u003cp\u003eThe product contains \u003cem\u003ePerfectStart\u003c\/em\u003e\u003csup\u003e®\u003c\/sup\u003e Fast Taq DNA Polymerase, optimized dual-cation buffer, Influor Green I, dNTPs, PCR enhancer, PCR stabilizer and \u003cspan\u003etracer dye\u003c\/span\u003e. Three monoclonal antibodies bind to the FastTaq DNA Polymerase engineered by directed evolution with high affinity, which are prepared by optimized process, effectively blocking DNA polymerase activity and inhibiting non-specific amplification at low temperature. The qPCR SuperMix is provided at 2× concentration and can be used at 1× concentration by adding template, primers, \u003cspan\u003eUniversal Passive Reference Dye\u003c\/span\u003e (optional) and nuclease-free water. \u003cspan\u003eThis product uses the principle of mixing yellow and blue to generate green color to track the pipetting process. The qPCR SuperMix contains a yellow dye, and 10×Sample Buffer contains a blue dye. Adding template diluted in Sample Buffer with a brighter color (bright blue) to the qPCR SuperMix (light yellow) will show an obvious color change from yellow to green with easy naked-eye visualization which is more effective in avoiding pipetting errors.\u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv class=\"product-content-sub-title\"\u003eFeatures\u003c\/div\u003e\n\u003cp\u003e•\u003cem\u003e PerfectStart\u003c\/em\u003e\u003csup\u003e®\u003c\/sup\u003e Fast Taq DNA Polymerase: fast and efficient; hot-start and blocking by 3 antibodies; high specificity, sensitivity and amplification efficiency; applicable to a wide range of species.\u003c\/p\u003e\n\u003cp\u003e• Dual-cation buffer enhances specificity and reduces primer-dimer formation.\u003c\/p\u003e\n\u003cp\u003e• Universal Passive Reference Dye for different instruments to correct differences in fluorescence detection between wells due to slight variations in reaction volume or differences in well position.\u003c\/p\u003e\n\u003cp\u003e• Contains tracer dye to avoid pipetting errors-a qPCR traffic-like style signal system to keep track of pipetting progress-see the results on how MixSure works in a 96-well plate.\u003c\/p\u003e\n\u003cdiv class=\"product-content-sub-title\"\u003eStorage\u003c\/div\u003e\nat -18℃ or below in dark for two years\n\u003cdiv class=\"product-content-sub-title\"\u003eShipping\u003c\/div\u003e\nDry ice(-70°C)\u003c\/div\u003e\n\u003cdiv class=\"region-title\"\u003eProduct Composition\u003c\/div\u003e\n\u003cdiv class=\"text\"\u003e\n\u003ctable border=\"1\" width=\"762\"\u003e\n\u003ctbody\u003e\n\u003ctr class=\"firstRow\"\u003e\n\u003ctd\u003e\n\u003cp\u003eComponent\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003eAQ621-01\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" valign=\"null\" width=\"null\"\u003eAQ621-02\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" valign=\"null\" width=\"null\"\u003eAQ621-03\u003c\/td\u003e\n\u003ctd\u003eAQ621-04\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e2×\u003cem\u003ePerfectStart\u003c\/em\u003e\u003csup\u003e®\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003eVisual Green qPCR SuperMix\u003c\/td\u003e\n\u003ctd\u003e1 ml\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" valign=\"null\" width=\"null\"\u003e5×1 ml\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" valign=\"null\" width=\"null\"\u003e15×1 ml\u003c\/td\u003e\n\u003ctd\u003e25×1 ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e10×Sample Buffer\u003c\/td\u003e\n\u003ctd\u003e200 μl\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" valign=\"null\" width=\"null\"\u003e1 ml\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" valign=\"null\" width=\"null\"\u003e3×1 ml\u003c\/td\u003e\n\u003ctd\u003e5×1 ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eUniversal Passive Reference Dye (50×)\u003c\/td\u003e\n\u003ctd\u003e40 μl\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" valign=\"null\" width=\"null\"\u003e200 μl \u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" valign=\"null\" width=\"null\"\u003e600 μl\u003c\/td\u003e\n\u003ctd\u003e1 ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eNuclease-free Water\u003c\/td\u003e\n\u003ctd\u003e1 ml\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" valign=\"null\" width=\"null\"\u003e5 ml\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" valign=\"null\" width=\"null\"\u003e3×5 ml\u003c\/td\u003e\n\u003ctd\u003e5×5 ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003c\/div\u003e","brand":"GeneBio Systems","offers":[{"title":"5mL","offer_id":46787666608228,"sku":"AQ621-01","price":397.84,"currency_code":"CAD","in_stock":true},{"title":"25mL","offer_id":46787666640996,"sku":"AQ621-02","price":1864.64,"currency_code":"CAD","in_stock":true},{"title":"50mL","offer_id":46787666673764,"sku":"AQ621-03","price":3182.7,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/1773626520146-mixsure_qPCR_signal_system.jpg?v=1774287637","url":"https:\/\/www.genebiosystems.com\/products\/mixsure-2-perfectstart-r-green-qpcr-supermix","provider":"GeneBio ","version":"1.0","type":"link"}