{"product_id":"il10-elisa-kit-for-mouse","title":"IL10 ELISA kit (Mouse)","description":"\u003cp\u003e\u003cb\u003eSize\u003c\/b\u003e: 96Tests\u003c\/p\u003e\u003cp\u003e\u003cb\u003e# of Times Cited in literature\u003c\/b\u003e: 124\u003c\/p\u003e\u003cp\u003e\u003cb\u003ePrepare Time\u003c\/b\u003e: 1-3 days(please inquire for mutiple units)\u003c\/p\u003e\u003cp\u003e\u003cb\u003eTarget Name\u003c\/b\u003e: IL10\u003c\/p\u003e\u003cp\u003e\u003cb\u003eTarget Full Name\u003c\/b\u003e: Interleukin 10\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAlternative Names\u003c\/b\u003e: CSIF; IL10A; TGIF; Cytokine Synthesis Inhibitory Factor\u003c\/p\u003e\u003cp\u003e\u003cb\u003eTarget Species\u003c\/b\u003e: Mouse\u003c\/p\u003e\u003cp\u003e\u003cb\u003eUniprot\u003c\/b\u003e: P18893\u003c\/p\u003e\u003cp\u003e\u003cb\u003eGene ID\u003c\/b\u003e: 16153\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFeatured Series\u003c\/b\u003e: SE kit\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFeatured Series Function\u003c\/b\u003e: Detects protein (regular version)\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSpecificity\u003c\/b\u003e: Reactive with Mouse IL10 \/ Interleukin 10\u003c\/p\u003e\u003cp\u003e\u003cb\u003eMethod\u003c\/b\u003e: Colormetric\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDetection principle\u003c\/b\u003e: Double-antibody Sandwich\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDetection\nrange\u003c\/b\u003e: 7.8-500pg\/mL\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSensitivity\u003c\/b\u003e: 2.9pg\/mL\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAssay Time\u003c\/b\u003e: 3h\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSample Size\u003c\/b\u003e: 100uL\u003c\/p\u003e\u003cp\u003e\u003cb\u003eRecommended\/Predicted\nSample Types\u003c\/b\u003e: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and other Biological Fluids\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAssay Precision\u003c\/b\u003e: Intra-Assay: CV\u0026lt;10%, Inter-Assay: CV\u0026lt;12%\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReproducibility test menthod\u003c\/b\u003e: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 10 (IL10) were tested 20 times on one plate, respectively.\nInter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 10 (IL10) were tested on 3 different plates, 8 replicates in each plate.\nCV(%) = SD\/meanX100\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage\u003c\/b\u003e: 4°C for 1 month\/ -20°C for long-term(One year within shelf life)\u003c\/p\u003e\u003cp\u003e\u003cb\u003eShelf-life\u003c\/b\u003e: 12 months\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSpecificity\u003c\/b\u003e: This assay has high sensitivity and excellent specificity for detection of Interleukin 10 (IL10).\nNo significant cross-reactivity or interference between Interleukin 10 (IL10) and analogues was observed.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStability\u003c\/b\u003e: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.\nTo minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAssay procedure summary\u003c\/b\u003e: 1. Prepare all reagents, samples and standards;\n2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;\n3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;\n4. Aspirate and wash 3 times;\n5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;\n6. Aspirate and wash 5 times;\n7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;\n8. Add 50µL Stop Solution. Read at 450nm immediately.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eTest principle\u003c\/b\u003e: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 10 (IL10). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 10 (IL10). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 10 (IL10), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 10 (IL10) in the samples is then determined by comparing the O.D. of the samples to the standard curve.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eResearch Area\u003c\/b\u003e: Cytokine;Infection immunity;\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReferences Citing This Product\u003c\/b\u003e: \u003ca href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/23498797\"\u003eOptimal formation of genetically modified and functional pancreatic islet spheroids by using hanging-drop strategy\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/26352932\"\u003eEfficacy of Albendazole-Chitosan Microsphere-based Treatment for Alveolar Echinococcosis in Mice\u003c\/a\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/25912691\"\u003eTrypanosoma cruzi infection and benznidazole therapy independently stimulate oxidative status and structural pathological remodeling of the liver tissue in mice\u003c\/a\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/26695151\"\u003eHypoxia-induced secretion of IL-19 from adipose-derived mesenchymal stem cell promotes growth and cancer stem cell properties of Burkitt lymphoma\u003c\/a\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/26105114\"\u003eModulation of oxidative and inflammatory cardiac response by nonselective 1‐and 5‐cyclooxygenase inhibitor and benznidazole in mice\u003c\/a\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/27473983\"\u003eA chimeric protein composed of the binding domains ofClostridium perfringens phospholipase C and Trueperella pyogenes pyolysin induces partial immunoprotection in a mouse model\u003c\/a\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/27064683\"\u003eLipopolysaccharide Cross-Tolerance Delays Platelet-Activating Factor-Induced Sudden Death in Swiss Albino Mice: Involvement of Cyclooxygenase in Cross-Tolerance\u003c\/a\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"http:\/\/www.tandfonline.com\/doi\/abs\/10.1080\/09540105.2017.1306494\"\u003eInfluences of Hyriopsis cumingii polysaccharides on mice immunosignaling molecules and T lymphocyte differentiation\u003c\/a\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/28134069\"\u003eNonsteroidal anti-inflammatory is more effective than anti-oxidant therapy in counteracting oxidative\/nitrosative stress and heart disease in T. cruzi-infected mice.\u003c\/a\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/28250847\"\u003eOral administration of Clostridium butyricum CGMCC0313‐1 reduces ovalbumin‐induced allergic airway inflammation in mice\u003c\/a\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"http:\/\/dx.doi.org\/10.1590\/s0100-736x2017000900013%20\"\u003eEfeito protetor do β-glucano e glutamina em lesões intestinais e imunológicas induzidas por ciratabina (Ara-C) em camundongos\u003c\/a\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"http:\/\/downloads.hindawi.com\/journals\/ecam\/aip\/1758383.pdf\"\u003eBuShenKangShuai tablet reduces atherosclerotic lesion by improving blood lipids metabolism and inhibiting inflammatory response via TLR4 and NF-κB signaling pathway\u003c\/a\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"https:\/\/www.nature.com\/articles\/s41598-017-01335-w?WT.feed_name=subjects_pharmacology\u0026amp;error=cookies_not_supported\"\u003eA Systems Pharmacology Approach to Determine Active Compounds and Action Mechanisms of Xipayi KuiJie’an enema for Treatment of\u003c\/a\u003e","brand":"GeneBio Systems","offers":[{"title":"Default Title","offer_id":48696684904548,"sku":"SEA056Mu","price":1058.4,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/no_image_default_image-jpeg_b9393bee-5257-4c45-8ec8-919266810dbc.jpg?v=1783134057","url":"https:\/\/www.genebiosystems.com\/products\/il10-elisa-kit-for-mouse","provider":"GeneBio ","version":"1.0","type":"link"}