{"product_id":"human-protein-s100-a5-s100a5-elisa-kit","title":"Human Protein S100-A5 (S100A5) ELISA Kit","description":"\u003cp\u003e\u003cb\u003eSpecies Reactivity\u003c\/b\u003e:Human (Homo sapiens)\u003c\/p\u003e\u003cp\u003e\u003cb\u003eUniProt\u003c\/b\u003e:P33763\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAbbreviation\u003c\/b\u003e:S100A5\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAlternative Names\u003c\/b\u003e:S100D; S100 calcium-binding protein A5\u003c\/p\u003e\u003cp\u003e\u003cb\u003eApplication\u003c\/b\u003e:ELISA\u003c\/p\u003e\u003cp\u003e\u003cb\u003eRange\u003c\/b\u003e:0.156-10 ng\/mL\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSensitivity\u003c\/b\u003e:0.042 ng\/mL\u003c\/p\u003e\u003cp\u003e\u003cb\u003eIntra-AssayCV\u003c\/b\u003e:?6.3%\u003c\/p\u003e\u003cp\u003e\u003cb\u003eInter-AssayCV\u003c\/b\u003e:?9.9%\u003c\/p\u003e\u003cp\u003e\u003cb\u003eRecovery\u003c\/b\u003e:1.04\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSample Type\u003c\/b\u003e:Serum, Plasma, Other biological fluids\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDetection Method\u003c\/b\u003e:Sandwich\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAnalysis Method??\u003c\/b\u003e:Quantitive\u003c\/p\u003e\u003cp\u003e\u003cb\u003eTest principle\u003c\/b\u003e:This assay employs a two-site sandwich ELISA to quantitate S100A5 in samples. An antibody specific for S100A5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyS100A5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for S100A5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of S100A5 bound in the initial step. The color development is stopped and the intensity of the color is measured.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Overview\u003c\/b\u003e:S100 calcium binding protein A5 is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and\/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21.\nThis protein has a Ca2 affinity 20- to 100-fold higher than the other S100 proteins studied under identical conditions. This protein also binds Zn2 and Cu2 , and Cu2 strongly which impairs the binding of Ca2 . This protein is expressed in very restricted regions of the adult brain.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStability\u003c\/b\u003e:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. \nThe loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"Default Title","offer_id":39788400476260,"sku":"AE20906HU","price":1436.18,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/no_image_default_image-jpeg_60636b3f-bc85-4dc9-b84f-360c045555cb.jpg?v=1660517984","url":"https:\/\/www.genebiosystems.com\/products\/human-protein-s100-a5-s100a5-elisa-kit","provider":"GeneBio ","version":"1.0","type":"link"}