{"product_id":"human-il-12p40-interleukin-12-p40-elisa-kit","title":"Human IL-12p40(Interleukin 12 p40) ELISA Kit","description":"\u003cp\u003e\u003cb\u003eSize\u003c\/b\u003e:96T\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReactivity\u003c\/b\u003e:Human\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAlternative Names\u003c\/b\u003e:IL-12B; p40; IL12B; CLMF2; NKSF2; Natural Killer Cell Stimulatory Factor 2; Cytotoxic Lymphocyte Maturation Factor 2; Interleukin 23, P40 Subunit; Interleukin 12B\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAssay Type\u003c\/b\u003e:Sandwich\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSensitivity\u003c\/b\u003e:2.8 pg\/mL\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStandard\u003c\/b\u003e:500 pg\/mL\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDetection range\u003c\/b\u003e:7.82-500 pg\/mL\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSample type\u003c\/b\u003e:Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAssay length\u003c\/b\u003e:3.5h\u003c\/p\u003e\u003cp\u003e\u003cb\u003eResearch Area\u003c\/b\u003e:Cytokine;Infection immunity;Immune molecule;\u003c\/p\u003e\u003cp\u003e\u003cb\u003eTest principle\u003c\/b\u003e:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL-12p40. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IL-12p40. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IL-12p40, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL-12p40 in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eUniProt ID\u003c\/b\u003e:P29460\u003c\/p\u003e","brand":"GeneBio Systems","offers":[{"title":"Default Title","offer_id":48645051220068,"sku":"ELK2263","price":961.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/no_image_default_image-jpeg_d65077b4-3e64-42f4-841b-ecd38e67a27a.jpg?v=1782664815","url":"https:\/\/www.genebiosystems.com\/products\/human-il-12p40-interleukin-12-p40-elisa-kit","provider":"GeneBio ","version":"1.0","type":"link"}