{"product_id":"human-gasdermin-d-gsdmd-elisa-kit","title":"Human Gasdermin D, GSDMD ELISA Kit","description":"\u003cp\u003e\u003cb\u003eSize\u003c\/b\u003e:96 Tests-1 Kit\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBackground\u003c\/b\u003e:[Gasdermin-D]: Precursor of a pore-forming protein that plays a key role in host defense against pathogen infection and danger signals (PubMed: 26375003, PubMed: 26375259, PubMed: 27281216). This form constitutes the precursor of the pore-forming protein: upon cleavage, the released N-terminal moiety (Gasdermin-D, N-terminal) binds to membranes and forms pores, triggering pyroptosis (PubMed: 26375003, PubMed: 26375259, PubMed: 27281216). [Gasdermin-D, N-terminal]: Promotes pyroptosis in response to microbial infection and danger signals (PubMed: 26375003, PubMed: 26375259, PubMed: 27418190, PubMed: 28392147, PubMed: 32820063). Produced by the cleavage of gasdermin-D by inflammatory caspases CASP1, CASP4 or CASP5 in response to canonical, as well as non-canonical (such as cytosolic LPS) inflammasome activators (PubMed: 26375003, PubMed: 26375259, PubMed: 27418190). After cleavage, moves to the plasma membrane where it strongly binds to inner leaflet lipids, including monophosphorylated phosphatidylinositols, such as phosphatidylinositol 4-phosphate, bisphosphorylated phosphatidylinositols, such as phosphatidylinositol (4,5)-bisphosphate, as well as phosphatidylinositol (3,4,5)-bisphosphate, and more weakly to phosphatidic acid and phosphatidylserine (PubMed: 27281216, PubMed: 29898893). Homooligomerizes within the membrane and forms pores of 10-15 nanometers (nm) of inner diameter, allowing the release of mature IL1B and triggering pyroptosis (PubMed: 27418190, PubMed: 27281216, PubMed: 29898893). Exhibits bactericidal activity (PubMed: 27281216). Gasdermin-D, N-terminal released from pyroptotic cells into the extracellular milieu rapidly binds to and kills both Gram-negative and Gram-positive bacteria, without harming neighboring mammalian cells, as it does not disrupt the plasma membrane from the outside due to lipid-binding specificity (PubMed: 27281216). Under cell culture conditions, also active against intracellular bacteria, such as Listeria monocytogenes (By similarity). Also active in response to MAP3K7\/TAK1 inactivation by Yersinia toxin YopJ, which triggers cleavage by CASP8 and subsequent activation (By similarity). Strongly binds to bacterial and mitochondrial lipids, including cardiolipin (PubMed: 27281216). Does not bind to unphosphorylated phosphatidylinositol, phosphatidylethanolamine nor phosphatidylcholine (PubMed: 27281216).\u003c\/p\u003e\u003cp\u003e\u003cb\u003eResearch_area\u003c\/b\u003e:Others\u003c\/p\u003e\u003cp\u003e\u003cb\u003eApplications\u003c\/b\u003e:ELISA\u003c\/p\u003e\u003cp\u003e\u003cb\u003eTarget_protein\u003c\/b\u003e:GSDMD\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReactivity\u003c\/b\u003e:Human\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAssay type\u003c\/b\u003e:Sandwich\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSensitivity\u003c\/b\u003e:0.017ng\/ml\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDetection range\u003c\/b\u003e:0.05-15ng\/ml\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSample type\u003c\/b\u003e:Serum, plasma or other biological fluids\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFull product name\u003c\/b\u003e:Gasdermin D\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSpecies\u003c\/b\u003e:Human\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage\u003c\/b\u003e:2-8ºC\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAssay time\u003c\/b\u003e:1h 30m\u003c\/p\u003e\u003cp\u003e\u003cb\u003eUniProt accession\u003c\/b\u003e:P57764\u003c\/p\u003e\u003cp\u003e\u003cb\u003eMASS(Da)\u003c\/b\u003e:52,801\u003c\/p\u003e\u003cp\u003e\u003cb\u003eGeneID\u003c\/b\u003e:79792\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSynonyms\u003c\/b\u003e:Gasdermin domain-containing protein 1;Gasdermin-D;Gasdermin-D, C-terminal;Gasdermin-D, N-terminal;GSDMD;GSDMD-CT;GSDMD-NT;hGSDMD-CTD;hGSDMD-NTD;\u003c\/p\u003e\u003cp\u003e\u003cb\u003eGene names\u003c\/b\u003e:GSDMD\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStandard solution\u003c\/b\u003e:16ng\/ml\u003c\/p\u003e","brand":"GeneBio Systems","offers":[{"title":"Default Title","offer_id":48648177680484,"sku":"E6838Hu","price":1025.92,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/no_image_default_image-jpeg_6b8ff521-1b94-402a-9fa1-67052e49ae44.jpg?v=1782711124","url":"https:\/\/www.genebiosystems.com\/products\/human-gasdermin-d-gsdmd-elisa-kit","provider":"GeneBio ","version":"1.0","type":"link"}