{"product_id":"gpr177-antibody-cat-fnab03601","title":"GPR177 antibody , Cat. #  FNab03601","description":"\u003cp\u003e\u003cb\u003eSize\u003c\/b\u003e:100ug\u003c\/p\u003e\u003cp\u003e\u003cb\u003ePurification\u003c\/b\u003e:Immunogen affinity purified\u003c\/p\u003e\u003cp\u003e\u003cb\u003eForm\u003c\/b\u003e:liquid\u003c\/p\u003e\u003cp\u003e\u003cb\u003ePurity\u003c\/b\u003e:?95% as determined by SDS-PAGE\u003c\/p\u003e\u003cp\u003e\u003cb\u003eHost\u003c\/b\u003e:Rabbit\u003c\/p\u003e\u003cp\u003e\u003cb\u003eClonality\u003c\/b\u003e:polyclonal\u003c\/p\u003e\u003cp\u003e\u003cb\u003eClone ID\u003c\/b\u003e:\u003c\/p\u003e\u003cp\u003e\u003cb\u003eIsotype\u003c\/b\u003e:IgG\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage\u003c\/b\u003e:PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20? for 12 months(Avoid repeated freeze \/ thaw cycles.)\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBackground\u003c\/b\u003e:GPR177, also known as Wntless (WLS) or Evi, is an evolutionarily conserved transmembrane protein necessary for Wnt protein secretion. GPR177 localizes to compartments of the secretory pathway, including the ER, Golgi apparatus, endosomes, and plasma membrane. GPR177 is overexpressed in several cancer types including glioma and gastric cancer. Several isoforms of GPR177 exist due to the alternative splicing, with predicted MW around 62 and 51 kDa, respectively. The sequence can be processed by removing the signal peptide, which generates a mature form of 45-46 kDa. In addition, slow migragrating band above 62 kDa can also be detected because GPR177 is modified by N-linked glycosylation (PMID: 20549736).\u003c\/p\u003e\u003cp\u003e\u003cb\u003eImmunogen\u003c\/b\u003e:G protein-coupled receptor 177\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSynonyms\u003c\/b\u003e:C1orf139, EVI, G protein coupled receptor 177, GPR177, Protein wntless homolog, Sprinter, WLS, Wntless\u003c\/p\u003e\u003cp\u003e\u003cb\u003eObserved MW\u003c\/b\u003e:46-62 kDa \u003c\/p\u003e\u003cp\u003e\u003cb\u003eUniprot ID\u003c\/b\u003e:Q5T9L3\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReactivity\u003c\/b\u003e:Human, Mouse, Rat\u003c\/p\u003e\u003cp\u003e\u003cb\u003eTested Application\u003c\/b\u003e:ELISA, WB, IHC, IP\u003c\/p\u003e\u003cp\u003e\u003cb\u003eRecommended dilution\u003c\/b\u003e:WB: 1:500-1:1000; IP: 1:500-1:1000; IHC: 1:20-1:200\u003c\/p\u003e\u003cp\u003e\u003cb\u003eGene ID\u003c\/b\u003e:79971\u003c\/p\u003e\u003cp\u003e\u003cb\u003eResearch Area\u003c\/b\u003e:Signal Transduction\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"Default Title","offer_id":40499569000548,"sku":"FNab03601","price":564.41,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/no_image_default_image-jpeg_684040b0-1c33-4e0e-8961-59030fcb8269.jpg?v=1660503201","url":"https:\/\/www.genebiosystems.com\/products\/gpr177-antibody-cat-fnab03601","provider":"GeneBio ","version":"1.0","type":"link"}