{"product_id":"gadd34-antibody-cat-fnab03293","title":"GADD34 antibody , Cat. #  FNab03293","description":"\u003cp\u003e\u003cb\u003eSize\u003c\/b\u003e:100ug\u003c\/p\u003e\u003cp\u003e\u003cb\u003ePurification\u003c\/b\u003e:Immunogen affinity purified\u003c\/p\u003e\u003cp\u003e\u003cb\u003eForm\u003c\/b\u003e:liquid\u003c\/p\u003e\u003cp\u003e\u003cb\u003ePurity\u003c\/b\u003e:?95% as determined by SDS-PAGE\u003c\/p\u003e\u003cp\u003e\u003cb\u003eHost\u003c\/b\u003e:Rabbit\u003c\/p\u003e\u003cp\u003e\u003cb\u003eClonality\u003c\/b\u003e:polyclonal\u003c\/p\u003e\u003cp\u003e\u003cb\u003eClone ID\u003c\/b\u003e:\u003c\/p\u003e\u003cp\u003e\u003cb\u003eIsotype\u003c\/b\u003e:IgG\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage\u003c\/b\u003e:PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20? for 12 months(Avoid repeated freeze \/ thaw cycles.)\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBackground\u003c\/b\u003e:GADD34, also named PPP1R15A, belongs to the PPP1R15 family. GADD34 can be triggered as a direct target of activating transcription factor4(ATF4) under ER stress, it plays a pivotal role in the recovery of cells from shut-down of translation induced by ER stress. It recruits the serine\/threonine-protein phosphatase(PP1) to dephosphorylate the translation initiation factor eIF2alpha, thereby reversing the shut-off of protein synthesis initiated by stress-inducible kinases and facilitating recovery of cells from stress. GADD34 down-regulates the TGF-beta signaling pathway by promoting dephosphorylation of TGFB1 via PP1. It may also promote apoptosis by inducing TP53 phosphorylation on 'Ser-15'. Starvation-induced expression of GADD34 reduced mTOR activity and induced autophagy in wild-type mice, but not in GADD34 KO mice. Molecular weight of GADD34 appears to be about 100 kDa which was confirmed in GADD34 KO mice and drug treated cells, despite the predicated MW of 73 kDa, and this antibody primarily recongize the 100 kDa band.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eImmunogen\u003c\/b\u003e:protein phosphatase 1, regulatory(inhibitor) subunit 15A\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSynonyms\u003c\/b\u003e:GADD34, growth arrest and DNA damage-inducible protein 34, PPP1R15A\u003c\/p\u003e\u003cp\u003e\u003cb\u003eObserved MW\u003c\/b\u003e:100 kDa \u003c\/p\u003e\u003cp\u003e\u003cb\u003eUniprot ID\u003c\/b\u003e:O75807\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReactivity\u003c\/b\u003e:Human, Mouse, Rat\u003c\/p\u003e\u003cp\u003e\u003cb\u003eTested Application\u003c\/b\u003e:ELISA, WB, IHC, IF\u003c\/p\u003e\u003cp\u003e\u003cb\u003eRecommended dilution\u003c\/b\u003e:WB: 1:200-1:1000; IHC: 1:20-1:200; IF: 1:20-1:200\u003c\/p\u003e\u003cp\u003e\u003cb\u003eGene ID\u003c\/b\u003e:\u003c\/p\u003e\u003cp\u003e\u003cb\u003eResearch Area\u003c\/b\u003e:Epigenetics\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"Default Title","offer_id":40499546423396,"sku":"FNab03293","price":564.41,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/no_image_default_image-jpeg_bb23ba66-ac66-49b3-abb6-d7a1a8695cb1.jpg?v=1660502722","url":"https:\/\/www.genebiosystems.com\/products\/gadd34-antibody-cat-fnab03293","provider":"GeneBio ","version":"1.0","type":"link"}