1) Unlike other dyes, DyeNA-View does not alter the mobility of DNA on gel, so you can determine your the size of your DNA accurately!
2) This DNA Gel electrophoresis dye works for UV transilliminators and Blue LED transilluminators, and UV and LED based gel doc systems. Yes, including your Bio-Rad Imaging systems.
3) DyeNA-View is a safer dye than is Ethidium Bromide.
It emits green fluorescence with broad spectral wavelength range. This new stain has two fluorescence excitation maximas when bound to nucleic acid, one centered at 268 nm and another at 294 nm. In addition, it has one visible excitation at 491 nm. This is why this dye has the dual compatibility with transilluminators/gel doc systems based on UV or Blue Light excitation.
The Fluorescence emission of DyeNA-View™ bound to DNA is centered at 530 nm. These spectral characteristics make DyeNA-View™ I compatible with a wide variety of gel reading instruments, ranging from those with ultraviolet epi- and transillumination to argon-ion laser and mercury-arc lamp excitation gel scanners.
DyeNA-View™ products are non-carcinogenic by the Ames-test. The results are negative in both the mouse marrow chromophilous erythrocyte micronucleus and mous spermary spermatocyte chromosomal aberration tests. Although DyeNA-View™ Nucleic Acid stains are non-carcinogenic, it may cause skin and eye-irritations. Always wear gloves when working with the product.
1. Prepare 100mL of agarose gel solution (concentration from 0.8-2%) in a 250mL flask and mix thoroughly.
Place the flask in a microwave oven, heat until the solution is completely clear and no small floating
particles are visible (about 2-3 minutes).
2. Let agarose solution cool down to about 50 °C (about when you can comfortably keep your hand on the flask), about 5 mins.
3. Vortex the DyeNA-View™ tube for 2-3 seconds. Add 2-5µL of DyeNA-View™ to the gel solution. Swirl the flask gently to mix the stain solution into the molten agarose while avoiding bubbles.
4. Pour enough gel solution into the gel tray until the comb teeth are immersed at least about ¼-½ into the gel solution.
5. Allow the agarose gel to cool until solidified. Load samples on the gel and perform electrophoresis.
[Optional: For increased sensitivity, after gel is electrophoresed, perform a post stain rinse by
incubating the gel in distilled water for 5 min.]
6. View the DNA bands under UV/Blue LED illumination.
A commercial 100 bp ladder was loaded in 10 and 5 ul into each of two wells. RedSafe™ (FroggaBio) and DyeNA-View™ (GeneBio Systems, Inc.) were used according to instructions provided by the manufacturers.