{"product_id":"ddddk-tag-binds-to-flag-sequnence-rabbit-pab-antibody","title":"DDDDK-Tag(binds to flag sequnence) rabbit pAb","description":"\u003cp\u003e\u003cb\u003eSize\u003c\/b\u003e: 100μL\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSource\u003c\/b\u003e:Rabbit\u003c\/p\u003e\u003cp\u003e\u003cb\u003eApplications\u003c\/b\u003e:WB;ELISA;IP;IF\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReactivity\u003c\/b\u003e:Species independent\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDilution\u003c\/b\u003e:Western Blot: 1\/1000 - 1\/3000. ELISA: 1:5000-20000. Not yet tested in other applications.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eImmunogen\u003c\/b\u003e:DDDDK synthetic peptide conjugated to KLH.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage_stability\u003c\/b\u003e:-20°C\/1 year\u003c\/p\u003e\u003cp\u003e\u003cb\u003eClonality\u003c\/b\u003e:Polyclonal\u003c\/p\u003e\u003cp\u003e\u003cb\u003eIsotype\u003c\/b\u003e:IgG\u003c\/p\u003e\u003cp\u003e\u003cb\u003eConcentration\u003c\/b\u003e:1 mg\/ml\u003c\/p\u003e\u003cp\u003e\u003cb\u003eObserved_band(KD)\u003c\/b\u003e:\u003c\/p\u003e\u003cp\u003e\u003cb\u003eHuman_gene_id\u003c\/b\u003e:\u003c\/p\u003e\u003cp\u003e\u003cb\u003eHuman_swiss_prot_no\u003c\/b\u003e:\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSubcellular_location\u003c\/b\u003e:\u003c\/p\u003e\u003cp\u003e\u003cb\u003eOther_name\u003c\/b\u003e:DDDDK epitope tag; DDDDK epitope tag; DYKDDDDK epitope tag\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBackground\u003c\/b\u003e:The DYKDDDDK (FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal FLAG vectors provide an Ek cleavage site for removal of the fusion tag. The FLAG peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the FLAG peptide is more likely to be accessible to antibodies. A FLAG-tag can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization and so on.\u003c\/p\u003e","brand":"GeneBio Systems","offers":[{"title":"Default Title","offer_id":48647069401188,"sku":"ES1078","price":477.4,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/no_image_default_image-jpeg_008e9336-15fb-4fd2-a0e1-be53e0874c92.jpg?v=1782687687","url":"https:\/\/www.genebiosystems.com\/products\/ddddk-tag-binds-to-flag-sequnence-rabbit-pab-antibody","provider":"GeneBio ","version":"1.0","type":"link"}